Anti ampkα mab

Cell samples were collected in RIPA buffer after washing with cold PBS. Primary antibodies, anti-AMPKα mAb (Cat^#2793, Cell Signaling, USA), Phospho-AMPKα mAb (Cat^#2535, Cell Signaling, USA), iNOS mAb (MAB9502-SP, R&D systems, USA), anti-CREB mAb (Cat^#9197, Cell Signaling, USA), anti-phospho-CREB(ser133) mAb (Cat^#9198, Cell Signaling, USA) were used at 1:1000 dilution. Anti-mouse HRP was used as a secondary antibody at 1:2000 dilution (Cat^# ZB-2305, Zsbio, China). Anti-GAPDH (C1312, Applygen, China), anti-tubulin (Cat^# bsm-33034m, Bioss, China) were served as a loading controls.

Anti-MyD88 Ab, anti-phospho IRAK4 Ab, anti-IRAK4 mAb, anti-phospho p65 Ab, anti-phospho ULK1 mAb, anti-ULK1 mAb, anti-phospho AMPKα mAb, anti-AMPKα mAb, anti-LC3B mAb and horseradish peroxidase (HRP)-conjugated Rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-DYKDDDDK tag mAb, anti-tag antibody magnetic beads, DYKDDDDK peptide, coomassie G-250, compound C and HRP-conjugated anti-β-actin Ab were obtained from WAKO Pure Chemical Industries (Osaka, Japan). Lipopolysaccharide (LPS, Salmonella enterica serotype typhimurium) was purchased from Sigma Aldrich (St. Louis, MO, USA). Pam3CSK4, FLA-ST standard, FSL-1, ssRNA40/LyoVec and poly (I:C) HMW were obtained from InvivoGen (San Diego, CA, USA). Halt protease & phosphatase inhibitor single-use cocktail was purchased from Thermo Scientific. RIPA lysis buffer was obtained from Merck Millipore (Billerica, MA, USA). 3-methyladenine was purchased from Santa cruz Biotechnology (Dallas, TX, USA).