The LH surge paradigm was modeled after the injection paradigm used in Bosch et al. (41 (link)) Female mice were bilaterally ovariectomized and were allowed to recover for 5 days. After recovery, animals were subcutaneously injected at ZT10 with 0.25 µg of β-estradiol (EB; catalog no. E8875; Sigma-Aldrich. St Louis MO) dissolved in 100 µL of sesame oil (catalog no. S3547; Sigma-Aldrich). The following day, the female mice were subcutaneously injected with 1.5 µg of EB dissolved in 100 µL of sesame oil and soiled male bedding added to the cage. The following day (7 days after ovariectomy), the female mice were either euthanized or bled at ZT12 (lights off) and blood was collected for LH analysis. A mixed cohort of surge mice were sampled the following morning at ZT2 (AM) to establish negative feedback LH levels. We used a conservative surge threshold of the average AM LH level plus 2 SD (42 (link)).
β estradiol eb
Manufactured by Merck Group
β-estradiol (EB) is a chemical compound used in laboratory settings. It is a naturally occurring estrogen hormone. EB is commonly used as a reference standard in analytical procedures and research applications.
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2 protocols using β estradiol eb
1
Modeling the LH Surge in Mice
2
Induction of LH Surge in Ovariectomized Rodents
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The LH surge was induced using an injection paradigm described in Bosch et al. [31 (link)]. Briefly, animals underwent bilateral ovariectomy. Five days following the procedure, animals were given a subcutaneous injection of 0.25 µg of β-estradiol (EB; catalog no. E8875; Sigma-Aldrich) in 100 µL of sesame oil (catalog no. S3547; Sigma-Aldrich) at ZT10. On the following day, the animals were given a subcutaneous injection of 1.5 µg of EB in sesame oil (100 µL) at ZT10. On the following day, 7 days after ovariectomy, the animals were euthanized at ZT4 (1000 hours) or ZT12 (lights off; 1800 hours) and blood was collected for LH measurement.
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