In immunohistochemical staining, 10% formalin-fixed and paraffin-embedded tissues were processed into 3-µm-thick sections. The sections were reacted with 1,000-times-diluted anti-S100A16 polyclonal antibody (Abcam, Cambridge, UK) for 2 hours, anti-vimentin antibody (clone V9; DAKO, Glostrup, Denmark) for 2 hours, and 200-times-diluted anti-E-cadherin antibody (clone HECD-1; Takara, Kusatsu, Japan) for 1 hour at room temperature. The details of the procedure were described previously by our laboratory.17 (link)
Anti vimentin antibody clone v9
Manufactured by Agilent Technologies
Sourced in United Kingdom
Anti-vimentin antibody (clone V9) is a primary antibody that recognizes the vimentin protein, which is a type III intermediate filament found in various cell types. This antibody is commonly used for the identification and characterization of cells expressing vimentin, which is often associated with mesenchymal and undifferentiated cell types.
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Lab products found in correlation
2 protocols using anti vimentin antibody clone v9
1
Immunohistochemical Staining Protocol
2
Immunohistochemical Evaluation of Stem Cell Markers
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Ten-percent formalin-fixed and paraffin-embedded tissues were processed into 3-μm-thick sections. The sections were reacted with 100-times-diluted anti-nestin antibody (clone N1602, IBL; Takasaki, Japan) for 2 hrs at room temperature. The details of the procedure were described previously from our laboratory [7 (link)].
For ABCG2, E-cadherin, and Vimentin immunohistochemical staining, for antigen retrieval, the sections were autoclaved in 0.01 mol/L citrate buffer (pH 6.0) with 0.1% Tween 20 at 121°C for 10 min. The sections were reacted with 200-times-diluted anti-ABCG2 antibody (clone BXP-21, Abcam; Cambridge, UK) and anti-Vimentin antibody (clone V9, DAKO; Glostrup, Denmark) for 2 hrs at room temperature, and 200-times-diluted anti-E-cadherin antibody (clone HECD-1, Takara; Kusatsu, Japan) for 1 hr at room temperature.
For ABCG2, E-cadherin, and Vimentin immunohistochemical staining, for antigen retrieval, the sections were autoclaved in 0.01 mol/L citrate buffer (pH 6.0) with 0.1% Tween 20 at 121°C for 10 min. The sections were reacted with 200-times-diluted anti-ABCG2 antibody (clone BXP-21, Abcam; Cambridge, UK) and anti-Vimentin antibody (clone V9, DAKO; Glostrup, Denmark) for 2 hrs at room temperature, and 200-times-diluted anti-E-cadherin antibody (clone HECD-1, Takara; Kusatsu, Japan) for 1 hr at room temperature.
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