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Pcmv6 ac 3ddk empty vector

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The PCMV6-AC-3DDK empty vector is a cloning and expression vector that can be used for the expression of recombinant proteins in mammalian cell lines. It contains a cytomegalovirus (CMV) promoter for high-level protein expression and a C-terminal 3xDDK (FLAG) tag for detection and purification of the expressed protein.

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PCMV6 vector (40 citations) PCMV6 empty vector (14 citations) Empty vector (10 citations) PCMV6-AC vector (9 citations) PCMV6-AC-3DDK (2 citations)

2 protocols using pcmv6 ac 3ddk empty vector

1

Molecular Probes for Cellular Signaling

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A full-length human SMPDL3b with GFP tag (hSMPDL3b-pCMV6-AC-GFP; #RG217688), human caveolin-1 with GFP tag (hCav1-pCMV6-AC-GFP; #RG210274), human caveolin-1 with FLAG tag (hCav1-pCMV6-Myc-DDK; #RC210274), and pCMV6-AC-3DDK empty vector (#PS100057) were ordered from OriGene (MD, USA). A full-length human IR isoform A with FLAG tag (hIRA-FLAG-pRC/CMV) and a full-length human IR B with FLAG tag (hIRB-FLAG-pRC/CMV) plasmids were a kind gift from our collaborator Dr. Ingo Leibiger13 (link). A full-length human HA-tagged SMPDL3b plasmid (pTO-SP-HA-hSMPDL3b) and HA-tagged SMPDL3b plasmid with mutation in 135 histidine (pTO-SP-HA-hSMPDL3b-H135A) were a kind gift from our collaborator Dr. Leonhard Heinz4 (link).
Mitrofanova A., Mallela S.K., Ducasa G.M., Yoo T.H., Rosenfeld-Gur E., Zelnik I.D., Molina J., Varona Santos J., Ge M., Sloan A., Kim J.J., Pedigo C., Bryn J., Volosenco I., Faul C., Zeidan Y.H., Garcia Hernandez C., Mendez A.J., Leibiger I., Burke G.W., Futerman A.H., Barisoni L., Ishimoto Y., Inagi R., Merscher S, & Fornoni A. (2019). SMPDL3b modulates insulin receptor signaling in diabetic kidney disease. Nature Communications, 10, 2692.
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2

Plasmid Expression of SMPDL3b and CERK

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The HA-tagged human SMPDL3b plasmid (pTO-SP-HA-hSMPDL3b) and the HA-tagged SMPDL3b plasmid with a mutation at position 135 (pTO-SP-HA-hSMPDL3b-H135A) were a kind gift from our collaborator Dr. Leonhard Heinz [12 (link)]. A human FLAG-tagged CERK (#20454) plasmid was purchased from Addgene (MA, USA) and the pCMV6-AC-3DDK empty vector (#PS100057) and FLAG-tagged human SMPDL3b (#RC217688) plasmids from OriGene (MD, USA).
Mallela S.K., Mitrofanova A., Merscher S, & Fornoni A. (2019). Regulation of the amount of Ceramide-1-Phosphate synthesized in differentiated human podocytes. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864(12), 158517.
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