Penicillin steptomycin

HiPrep CM FF 16/10 column was purchased from GE Healthcare. SunFire C18 column (19×150 mm) was purchased from Waters. Penicillin-Steptomycin, MTT, dimethyl sulfoxide (DMSO), Diethylpyrocarbonate (DEPC) was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Fetal calf serum and Dulbecco's Modified Eagle's Medium (DMEM) culture medium supplemented with Glutamine were purchased from Gibco Invitrogen (Carlsbad, CA, USA). Bicinchoninic acid (BCA) protein assay kit was obtained from Thermo scientific (Pierce Inc., Rockford, IL, USA). Other commercially available chemicals and reagents were analytical grade. All reagents were prepared using deionized MilliQ water.

Hiprep Q FF 16/10 column was purchased from GE Healthcare (Uppsala, Sweden). Protein-PAKTM60 was purchased from Waters (Milford, MA, USA). Penicillin-steptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) were purchased from sigma (St. Louis, MO, USA), Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal calf serum were products of Gibco Invitrogen (Carlsbad, CA, USA). BCA (Bicinchoninic acid) protein assay kit was purchased from Thermo Scientific (Pierce Inc., Rockford, IL, USA). Integrin β1, FAK, p-FAK, AKT, p-AKT, ERK, p-ERK, MMP-2, MMP-9, GAPD Hantibodies were obtained from Cell Signaling technology (Beverly, MA, USA). Peroxidase-Conjugated Affiniure Goat Anti-Rabbit IgG (H + L) secondary antibodies were purchased from Origene (Rockville, FL, USA).