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Gapdh ab2302

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GAPDH (AB2302) is a monoclonal antibody that detects glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme involved in the glycolytic pathway. This antibody can be used for various laboratory applications, including immunoblotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (4 mentions) Ab94571, by Abcam (2 mentions) Ab58641, by Abcam (2 mentions) Ab55271, by Abcam (2 mentions) Pdhα pser293, by Merck Group (2 mentions) Aif sc 13116, by Santa Cruz Biotechnology (2 mentions) Ctp sc 86392, by Santa Cruz Biotechnology (2 mentions) Hif 1α, by Cell Signaling Technology (2 mentions) Actin, by Merck Group (2 mentions) Control immunoglobulin g, by R&D Systems (2 mentions) Ab175798, by Merck Group (2 mentions) Phosphothr202 tyr204 erk 9101, by Santa Cruz Biotechnology (2 mentions) Pin1 mab2294, by Merck Group (2 mentions) Cenpf ab 5, by Abcam (2 mentions) Cyclin b1 for, by Thermo Fisher Scientific (2 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Tau 5, by Lab Vision (1 mentions) Vincristine, by Merck Group (1 mentions) Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions) Plk1 thr210, by Cell Signaling Technology (1 mentions)

7 protocols using gapdh ab2302

1

Characterization of Alzheimer's Pathology

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The following antibodies were used: monoclonal anti-Aβ antibodies (mouse 4G8; Covance, Munich, Germany), monoclonal anti-neuronal nuclei antibody NeuN (mouse; Chemicon, Temecula, CA, USA), monoclonal anti-actin antibody (mouse; Calbiochem; Darmstadt, Germany), monoclonal anti-APP antibody (22C11; Millipore GmbH Schwalbach/Ts., Germany), monoclonal anti-synaptophysin antibody (mouse; Millipore), PSD-95 (7E3-1B8; mouse; Thermo Fisher Scientific, Rockford, IL, USA), monoclonal anti-Arc antibody (C-7; mouse; Santa Cruz Biotechnology, Dallas, TX, USA) and GAPDH (#AB2302; chicken; Millipore). The following anti-Tau antibodies were used: monoclonal phosphorylation independent Tau-5 (mouse; Labvision, Westinghouse, CA, USA), monoclonal AT8 (mouse; Thermo Fisher Scientific, Rockford, IL, USA), polyclonal pS199 (rabbit; Invitrogen, Carlsbad, CA, USA), and monoclonal PHF1 (mouse; a generous gift from Peter Davies, Albert Einstein College of Medicine, Bronx, NY, USA). As secondary antibodies, cyanine 3 (Cy3)-coupled anti-mouse (Dianova, Hamburg, Germany) and peroxidase-conjugated anti-mouse and anti-rabbit antibodies (Jackson Immuno Research, West Grove, PA, USA) were employed.
Hrynchak M.V., Rierola M., Golovyashkina N., Penazzi L., Pump W.C., David B., Sündermann F., Brandt R, & Bakota L. (2020). Chronic Presence of Oligomeric Aβ Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression. Frontiers in Synaptic Neuroscience, 12, 16.
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2

Quantifying Metabolic Enzyme Profiles

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Whole cells/spheroids or mitochondrial lysates were prepared in RIPA buffer and quantified using the BCA Protein Assay (Thermo Scientific). Proteins were separated on 4–20% SDS-PAGE gels, transferred to PVDF membranes, and probed with antibodies against IDH1 (ab94571), IDH2 (ab55271), IDH3 (ab58641) from Abcam, PDHα (#459400, Theromo), PDHα-pSer293 (AP1062), GAPDH (AB2302) from Millipore, PDK1 (#3820), Hif1α(#3716) from Cell Signaling, CTP (sc-86392), AIF (sc-13116) from Santa Cruz Biotechnology and Actin (A3853, Sigma).
Jiang L., Shestov A.A., Swain P., Yang C., Parker S.J., Wang Q.A., Terada L.S., Adams N.D., McCabe M.T., Pietrak B., Schmidt S., Metallo C.M., Dranka B.P., Schwartz B, & DeBerardinis R.J. (2016). Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature, 532(7598), 255-258.
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3

Synthesis and Evaluation of HDAC6/Tubulin Inhibitor MPT0B451

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MPT0B451, a dual HDAC6 and tubulin inhibitor was synthesized by Dr. Jing-Ping Liou (School of Pharmacy, College of Pharmacy, Taipei Medical University, Taiwan). Vincristine, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and propidium iodide (PI) were purchased from Sigma Chemical Co. (St. Louis, MO, United States). Primary antibodies against Caspase 8 (06-775), MPM2 (pSer/pThr) (05-368) and GAPDH (AB2302) were purchased from Millipore (Bedford, MA, United States). The antibodies against Acetyl-Histone H3 (Lys9) (#9649), Histone H3 (#9715), Acetyl-α-tubulin (#3971), α-tubulin (#2144), Cyclin B1(#4135), Aurora A (#14475), Aurora A (Thr288) (#3079), PLK1 (#4535), PLK1 (Thr210) (#9062), cleaved caspase-3 (#9664), PARP (#9542), and caspase 9 (#9508) were purchased from Cell Signaling Technology (Beverly, MA, United States). The antibodies against Cdc2 (SC-54) and cdc25c (SC-372) as well as the labeled secondary antibodies goat anti-rabbit IgG-HRP (SC-2004) and goat anti-mouse IgG-HRP (SC-2005) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States).
Wu Y.W., Hsu K.C., Lee H.Y., Huang T.C., Lin T.E., Chen Y.L., Sung T.Y., Liou J.P., Hwang-Verslues W.W., Pan S.L, & HuangFu W.C. (2018). A Novel Dual HDAC6 and Tubulin Inhibitor, MPT0B451, Displays Anti-tumor Ability in Human Cancer Cells in Vitro and in Vivo. Frontiers in Pharmacology, 9, 205.
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4

Quantifying Metabolic Enzyme Profiles

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Whole cells/spheroids or mitochondrial lysates were prepared in RIPA buffer and quantified using the BCA Protein Assay (Thermo Scientific). Proteins were separated on 4–20% SDS-PAGE gels, transferred to PVDF membranes, and probed with antibodies against IDH1 (ab94571), IDH2 (ab55271), IDH3 (ab58641) from Abcam, PDHα (#459400, Theromo), PDHα-pSer293 (AP1062), GAPDH (AB2302) from Millipore, PDK1 (#3820), Hif1α(#3716) from Cell Signaling, CTP (sc-86392), AIF (sc-13116) from Santa Cruz Biotechnology and Actin (A3853, Sigma).
Jiang L., Shestov A.A., Swain P., Yang C., Parker S.J., Wang Q.A., Terada L.S., Adams N.D., McCabe M.T., Pietrak B., Schmidt S., Metallo C.M., Dranka B.P., Schwartz B, & DeBerardinis R.J. (2016). Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature, 532(7598), 255-258.
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5

Antibody Catalog for Cell Signaling

Cited 1 time
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The HA antibody (12CA5) has been described.40 (link) The following antibodies were purchased. Cell Signaling (Leiden, The Netherlands): phosphoThr202/Tyr204-ERK (9101), Santa Cruz Biotechnology (Heidelberg, Germany): BRAF (C19), cyclin B1 for immunoblot (GNS1), FOXM1 for immunoprecipitation, immunoblot and immunofluorescence in vitro (MPP2; C-20 and H-92), control immunoglobulin G, R&D systems (Minneapolis, MN, USA): Pin1 (MAB2294), Sigma-Aldrich: Pin1 (WH0005300M1, St Louis, MO, USA), FLAG-M2 (F1804), Tubulin (T5168), Abcam: CENPF (Ab-5, Cambridge, UK) and FOXM1 for immunofluorescence in tissue (ab175798), Chemicon: GAPDH (AB2302, Merck Millipore, Billerica, MA, USA), Pierce: cyclin B1 for immunofluorescence (MA5-13128, Thermo Fisher Scientific, Waltham, MA, USA).
Kruiswijk F., Hasenfuss S.C., Sivapatham R., Baar M.P., Putavet D., Naipal K.A., van den Broek N.J., Kruit W., van der Spek P.J., van Gent D.C., Brenkman A.B., Campisi J., Burgering B.M., Hoeijmakers J.H, & de Keizer P.L. (2015). Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling. Oncogene, 35(17), 2166-2177.
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6

Antibody Catalog for Cell Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The HA antibody (12CA5) has been described.40 (link) The following antibodies were purchased. Cell Signaling (Leiden, The Netherlands): phosphoThr202/Tyr204-ERK (9101), Santa Cruz Biotechnology (Heidelberg, Germany): BRAF (C19), cyclin B1 for immunoblot (GNS1), FOXM1 for immunoprecipitation, immunoblot and immunofluorescence in vitro (MPP2; C-20 and H-92), control immunoglobulin G, R&D systems (Minneapolis, MN, USA): Pin1 (MAB2294), Sigma-Aldrich: Pin1 (WH0005300M1, St Louis, MO, USA), FLAG-M2 (F1804), Tubulin (T5168), Abcam: CENPF (Ab-5, Cambridge, UK) and FOXM1 for immunofluorescence in tissue (ab175798), Chemicon: GAPDH (AB2302, Merck Millipore, Billerica, MA, USA), Pierce: cyclin B1 for immunofluorescence (MA5-13128, Thermo Fisher Scientific, Waltham, MA, USA).
Kruiswijk F., Hasenfuss S.C., Sivapatham R., Baar M.P., Putavet D., Naipal K.A., van den Broek N.J., Kruit W., van der Spek P.J., van Gent D.C., Brenkman A.B., Campisi J., Burgering B.M., Hoeijmakers J.H, & de Keizer P.L. (2015). Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling. Oncogene, 35(17), 2166-2177.
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7

Multiparameter Western Blot Analysis

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Membranes were blotted with p-EGFRY1173 (Invitrogen, 44–794G), EGFR (SC-03), ERK2 (SC-154), p-PKCαS657 (SC-12356) (Santa Cruz Biotechnology), p-AKTS473 (9271S), AKT (4691S), p-ERKT202/Y204 (4370S), p-RPS6S235/236 (4858S), RPS6 (2217S), p-4EBP1T37/46 (2855S), 4EBP1 (9644S), p-PKC (pan) (βII Ser660) (9371S), PKCα (59754S), p-MARCKSS152/156 (2741S), MARCKS (5607S), p-STAT3Y705 (9145S), STAT3 (4904S), PARP (9532S), and JAK2 (3230S) (Cell Signaling), 4G10 (05–321), and GAPDH (AB2302) (EMD Millipore). Bound antibodies were detected with HRP-linked anti-mouse (DC-02L) or anti-rabbit IgG (DC-03L) (Calbiochem), followed by ECL (Amersham, RPN2106).
Wong R.A., Luo X., Lu M., An Z., Haas-Kogan D.A., Phillips J.J., Shokat K.M., Weiss W.A, & Fan Q.W. (2019). Cooperative blockade of PKCα and JAK2 drives apoptosis in glioblastoma. Cancer research, 80(4), 709-718.
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