A17604

The protein extraction and western blot were described in our previous study [30 (link)]. The specific primary antibodies are as follows: rabbit anti-ACSL4 (A6826, 1 : 1000, ABclonal, Wuhan, China), rabbit anti-LPCAT3 (A17604, 1 : 1000, ABclonal, Wuhan, China), rabbit anti-LOXs (A11504, 1 : 1000, ABclonal, Wuhan, China), rabbit anti-POR (YN1836, 1 : 1000, Immunoway, Plano, TX, USA), and mouse anti-GAPDH (RM2002, 1 : 10000, Beijing Ray Antibody Biotech, Beijing, China). The protein signals were detected after using the chemiluminescent substrate kit (Millipore, Darmstadt, Germany) and then analyzed by ImageJ software (NIH, Bethesda, MD, USA). Each experiment was repeated three times, and all protein expression was normalized to that of GAPDH.

DFO (USA, D9533) was purchased from Sigma-Aldrich. Recombinant mouse IL-1β (# 401-ML) and dimethylsulfoxide (DMSO) were purchased from R&D Systems (Minneapolis, USA). Erastin (USA, S7242) was obtained from Selleck and diluted in DMSO. An ROS assay kit (China, S0033) was provided by Beyotime. A C11 BODIPY Lipid Peroxidation Sensor (USA, D3861) was obtained from Thermo Fisher. A Micro Malondialdehyde (MDA) Assay Kit (China, BC0025) was purchased from Solarbio. A Micro Iron Concentration Assay Kit (China, TC1016) was provided by Leagene Biotechnology. Antibodies against GPX4 (UK, ab125066, diluted 1:5,000), SLC7A11 (UK, ab175186, diluted 1:5,000), ACSL4 (UK, ab155282, diluted 1:10,000), and P53 (UK, ab131442, diluted 1:1,000) were purchased from Abcam. Antibodies against Nrf2 (USA, 16396-1-AP, diluted 1:1,000), HO-1 (USA, 10701-1-AP, diluted 1:1,000), collagen II (USA, 28459-1-AP, diluted 1:2,000), MMP13 (USA, 18165-1-AP, diluted 1:2,000) and GAPDH (USA, 60004-1-Ig, diluted 1:1,000) were purchased from Proteintech. Antibodies against LOX15 (China, A6865, diluted 1:1,000) and lpcat3 (China, A17604, diluted 1:1,000) were purchased from Abclonal.