C57bl 6 tg zp3 cre 93knw j mice

C57BL/6J mice, C57BL/6-Tg(Zp3-cre)93Knw/J mice, Tg(Cd4-cre)1Cwi/BfluJ (CD4-cre) mice and C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I) mice were obtained from Jackson Laboratories. Mir31^fl/fl conditional knockout mice were produced on the C57BL/6J background at the Brigham and Women’s Hospital Transgenic Core Facility. Complete or conditional Mir31-knockout mice were produced by crossing Mir31^fl/fl mice to Zp3-cre (complete) or CD4-cre (conditional) knockout strains. All mice were maintained in specific-pathogen-free barrier facilities at the Dana-Farber Cancer Institute or at Charles River Laboratories. All experiments were performed in compliance with federal laws and institutional guidelines and were approved by the Animal Care and Use Committee of the Dana-Farber Cancer Institute (Protocol #04-103).

Animal use protocols were reviewed and approved by the University of Virginia Institutional Animal Care and Use Committee, and were in compliance with PHS and USDA guidelines for laboratory animal welfare. Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-Egfp)Luo}/J mice (The Jackson Laboratory), carrying a floxed gene for a membrane-targeted Tomato fluorescent protein followed by a gene for membrane-targeted enhanced green fluorescent protein (EGFP)^{25 (link)}, were crossed to C57BL/6^{-Tg(Zp3-cre)93Knw/}J mice (The Jackson Laboratory) to create mice with membrane-targeted EGFP in all cells^{26 (link)}. Tissue for imaging experiments was from female mice that were at least eight weeks in age.