Modular p chemistry analyser

Blood samples were taken in the fasting state between 08:00 and 10:00 hours and transported to the laboratory facility at room temperature or 4°C, depending on the sample requirements. On the same day, HbA_1c (EDTA-anticoagulated) was analysed using an NGSP-certified turbidimetric inhibition immunoassay on a Cobas Integra 800 CTS analyser (Roche Diagnostics Nederland, Almere, the Netherlands). Serum creatinine was measured on a Roche Modular P chemistry analyser (Roche, Basel, Switzerland) and renal function was calculated as estimated (e)GFR with the formula developed by the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) [31 (link)]. Total cholesterol and HDL-cholesterol were measured using an enzymatic colorimetric method, triacylglycerol using a colorimetric UV method, and LDL-cholesterol using an enzymatic method, on a Roche Modular P chemistry analyser (Roche). Fasting blood glucose was measured using a hexokinase method.

Blood samples were taken in the fasting state between 8:00 and 10:00 a.m. and transported to the laboratory facility at room temperature or 4°C, depending on the sample requirements. All measurements were performed the same day. Levels of TSH, FT4 and FT3 were assayed by electrochemiluminescence immunoassay (Roche Modular E170, Roche, Switzerland). Glycated haemoglobin (HbA1c, EDTA-anticoagulated) was analysed using an NGSP-certified turbidimetric inhibition immunoassay on a Cobas Integra 800 CTS analyser (Roche Diagnostics BV, Almere, The Netherlands). Serum creatinine was measured on a Roche Modular P chemistry analyser (Roche, Basel, Switzerland). Total and high density lipoprotein (HDL) cholesterol were measured using an enzymatic colorimetric method, triglycerides using a colorimetric UV method, and low density lipoprotein (LDL) cholesterol using an enzymatic method, on a Roche Modular P chemistry analyser (Roche, Basel, Switzerland). Fasting blood glucose was measured using a hexokinase method. The general Dutch population is iodine sufficient [26 (link)]. Anti-thyroid peroxidase antibody levels were not available.

Blood samples were collected between 8 and 10 a.m. after an overnight fast, directly into tubes containing heparin, and centrifuged. Total cholesterol (TC) and HDL-cholesterol (HDL-C) were measured with an enzymatic colorimetric method, triglycerides (TG) with UV colorometry, and LDL-cholesterol (LDL-C) using an enzymatic method (Roche Modular P chemistry analyser, Roche, Basel, Switzerland). HbA1c was measured with a turbidimetric inhibition immunoassay (Cobas Integra 800 CTS analyser, Roche Diagnostics Nederland BV, Almere, the Netherlands). Fasting blood glucose was measured using a hexokinase method. Levels of thyroid stimulating hormone (TSH), as well as free thyroxine (FT4) and free triiodothyronine (FT3) were assayed by electrochemiluminescent immunoassay (Roche Modular E170, Roche, Switzerland) [12 (link)]. Normal values for TSH are 0.4–4.5 mU/l, FT4 are 11–20 pmol/L, for FT3 4.4–6.7 pmol/l. The general Dutch population is iodine sufficient. Tests to measure anti-thyroid peroxidase antibody levels were not performed.