6- to 12-week-old female NSG mice (Jackson Laboratory, stock #05557) were sublethally irradiated (270 cGy) 24 h before tail-vein injection of 3 × 104 to 3 × 105 CD43+CD45+/− supernatant cells harvested and sorted at day 10 and 12 of iPSC differentiation. Bone marrow (BM) was collected 16 weeks post-transplantation and stained with human CD45-PE (clone HI30, BD Pharmingen). Animals were housed and handled in accordance with the guidelines set by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council (DHHS publication No. NIH 85–23), and the protocol was approved by the Animal Care and Use Committee of the NHLBI.
Human cd45 pe clone hi30
Manufactured by BD
Human CD45-PE (clone HI30) is a fluorescently labeled antibody that binds to the CD45 antigen expressed on the surface of human leukocytes. It can be used to identify and quantify various subsets of white blood cells in flow cytometry applications.
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Lab products found in correlation
2 protocols using human cd45 pe clone hi30
1
Engrafting iPSC-derived Cells in NSG Mice
2
Engrafting iPSC-derived Cells in NSG Mice
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6- to 12-week-old female NSG mice (Jackson Laboratory, stock #05557) were sublethally irradiated (270 cGy) 24 h before tail-vein injection of 3 × 104 to 3 × 105 CD43+CD45+/− supernatant cells harvested and sorted at day 10 and 12 of iPSC differentiation. Bone marrow (BM) was collected 16 weeks post-transplantation and stained with human CD45-PE (clone HI30, BD Pharmingen). Animals were housed and handled in accordance with the guidelines set by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council (DHHS publication No. NIH 85–23), and the protocol was approved by the Animal Care and Use Committee of the NHLBI.
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