Approximately, 1600 bp purified PCR products were sequenced with 18S rDNA region by using universal primers NS1 5' (GTA GTC ATA TGC TTG TCT C) 3' [42 ] and NS24 5' (AAA CCT TGT TAC GAC TTT TA) 3' [43 ], accomplished through Big Dye terminator cycle sequencing kit v.3.1. Both PCR sequencing and amplification was analysed by an automated DNA sequencing system (Applied Biosystems, Foster City, USA) at the Macrogen, Inc., Seoul, Korea.
Automated dna sequencing system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Automated DNA Sequencing System is a laboratory instrument designed for high-throughput DNA sequencing. It automates the process of determining the precise order of nucleotides within a DNA molecule. The system utilizes advanced optical detection and data analysis algorithms to generate sequencing data.
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Lab products found in correlation
2 protocols using automated dna sequencing system
1
Universal 18S rDNA Sequencing Protocol
2
Sequencing of 1600 bp PCR Product
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A purified PCR product of 1600 bp was sequenced with 18S rDNA region by utilizing universal primers ITS-1 (5'-TCC GTA GGT GAA CCT GCGG-3') and ITS-4 (5'-TCC TCC GCT TAT TGA TAT GC-3') [24 (link)]. A Big Dye terminator cycle sequencing kit v.3.1 was used for that purpose. Both PCR sequencing and amplification were analyzed by an automated DNA sequencing system (Applied Biosystems, Foster City, USA) at the Macrogen, Inc., Seoul, Korea. The obtained PCR product was initially sequenced and then subjected to a homology search by using online tool, BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi ).
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