Anti acetylated α tubulin clone 6 11b 1

The following tubulin antibodies were used: monoclonal anti-α-tubulin (Clone DM 1A) and anti-acetylated α-tubulin (Clone 6-11B-1) (Sigma-Aldrich), monoclonal anti-tyrosinated α-tubulin (YL1/2, Santa Cruz), and polyclonal anti-detyrosinated α-tubulin (Millipore). The following polyclonal antibodies were used: anti-GAPDH (HyTest), anti-Kif5A (Abcam), and anti-TTL (Proteintech Group). The following monoclonal antibodies were used: anti-β-catenin (Sigma-Aldrich), anti-KANK1 (Invitrogen), anti-paxillin (BD Transduction Laboratories), anti-sc35 (Abcam), and anti-vinculin (Sigma-Aldrich). The monoclonal antibody directed against sucrase-isomaltase (SI) (DRBB2/158) was generously provided by A. Quaroni. The plasmid mCherry-Vinculin-N-21 was a gift from Michael Davidson (Addgene plasmid #55160; RRID:Addgene_55160).

Zebrafish whole-mount immunofluorescence (IF) was performed as previously described^{33 (link)}. The following antibodies were used for IF: anti-CLIC5 (B-23; sc-133468; Santa Cruz Biotechnology; 1:10), anti-CLIC5 (A-11; sc-271863; Santa Cruz Biotechnology; 1:10), anti-γTubulin (clone GTU‐88; Sigma Aldrich; 1:5000), anti-GFP (ab13970; Abcam; 1:200), anti-acetylated αTubulin (clone 6-11B-1; Sigma Aldrich; 1:500) and anti-phospho-Ezrin (Thr567) (PA5-37763; Invitrogen; 1:200). Cy3 (1:1000) and Alexa-488/-546/-647 (1:1000) labelled secondary antibodies were purchased from Jackson Immunoresearch and Molecular Probes (Invitrogen), respectively. Immunoblotting (IB) was performed as previously described^{47 (link)}. The following antibodies were used for IB: anti-CLIC5 (B-23; sc-133468; Santa Cruz Biotechnology; 1:200), anti‐pERM (#3141; Cell Signaling; 1:1000), anti‐ERM (#3142; Cell Signaling; 1:1000), anti‐γTubulin (clone GTU‐88; Sigma Aldrich; 1:5000) and respective HRP‐conjugated antibodies (DAKO; 1:5000). Histological and transverse sectioning procedures were performed as previously described^{33 (link)}.

The following primary antibodies and dilutions were used: anti-Aβ42 rabbit polyclonal (1:5,000, Abcam); anti-Aβ (clone 6E10) mouse monoclonal (1:5,000, Signet); anti-human Aβ, N-terminus (clone 82E1) mouse monoclonal (1:6,000, IBL); anti-acetylated α-tubulin (clone 6-11B-1) mouse monoclonal (1:50,000, Sigma); anti-human amyloid precursor protein (hAPP) rabbit polyclonal (1:20,000, Sigma); anti-ATP synthase β (clone 10/ATP) mouse monoclonal (1:10,000, Brand BD Transduction Laboratories); anti-β-Actin (clone AC 74) mouse monoclonal (1:10,000, Sigma-Aldrich); anti-phospho-PHF-tau pSer202/Thr205 (clone AT8) mouse monoclonal (IHC 1:1,000, Cell Signaling); anti-PSD95 rabbit monoclonal (1:2,000, Cell signaling); anti-Somatostatin (SOM) goat polyclonal (1:1,000, Santa Cruz Biotechnology); anti-synaptophysin rabbit polyclonal (1:1,000, Abcam); anti-total tau (TAU46) mouse monoclonal (1:1,000, Cell Signaling) and anti-ubiquitin rabbit polyclonal (1:5,000, Dako).