Seahorse fluxpak calibration solution

We purchased tebufenpyrad (96% purity) from AK Scientific Inc. (Union City, CA), pyridaben (99.1% purity) from Chem Services (West Chester, PA), and rotenone (95–98% purity) and hydrogen peroxide (30 wt. % in H₂O) from Sigma (St. Louis, MO). DMSO was purchased from Fisher Scientific (Fair Law, NJ). We purchased RPMI 1640 media, fetal bovine serum (FBS), L-glutamine, penicillin, streptomycin and Sytox green nucleic acid fluorescence stain from Molecular Probes (Eugene, OR), the Muse® Count & Viability Assay Kit (Catalog # MCH100102) from EMD Millipore (Billerica, MA), and the 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H₂DCFDA) fluorescent probe and MitoTracker red CMXROS and MitoTracker green dyes from Invitrogen (Carlsbad, CA). The Cell Titer 96® AQueous Non-Radioactive Cell Proliferation assay kit and Cell Titer Glo Luminescent Cell Viability assay kit were bought from Promega (Madison, WI). The Aconitase assay kit was purchased from Abcam (Cambridge, MA). Oligomycin, hydrogen peroxide, carbonyl cyanide 4-trifluoromethoxy-phenylhydrazone (FCCP) and antimycin A were purchased from Sigma Aldrich (St. Louis, MO), and the Seahorse FluxPak calibration solution was bought from Seahorse Biosciences (Billerica, MA).

Dulbecco’s minimum essential medium (DMEM), penicillin, and streptomycin (PenStrep), L-glutamine, and trypsin-EDTA were purchased from Life Technologies (Carlsbad, CA). Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Flowery Branch, GA, catalog # S11150H and lot # A17002). Poly-D-Lysine (Sigma, catalog # P6407) was prepared and stored as 0.5 mg/mL stock at −20° C. Oligomycin, hydrogen peroxide, carbonyl cyanide 4-trifluoromethoxy-phenylhydrazone (FCCP) and antimycin A were purchased from Sigma Aldrich (St. Louis, MO), and the Seahorse FluxPak calibration solution was purchased from Seahorse Biosciences (Billerica, MA). Rhod-2 AM (ab142780) was purchased from Abcam (Cambridge, MA). Mitotraker green, Dulbecco’s phosphate buffer saline (DPBS), mitochondrial isolation kit for cultured cells (catalog # 89874), DNA purification kit (Thermo Fisher Scientific, catalog # K0512) were purchased from Thermo Fisher Scientific (Waltham, MA). IGEPAL CA630 (I3021) was purchased from Sigma. Mitoapocynin (MA) was procured from Dr. Balaraman Kalyanaraman (Medical College of Wisconsin, Milwaukee, WI), stock solution (10 mM/L in DMSO) was prepared by shaking vigorously and stored at - 20° C. MA was used (10 μM) as one of the co-treatments (Table 1).