Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap™, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
Sodium citrate
Manufactured by Mallinckrodt
Sourced in United Kingdom
Sodium citrate is a chemical compound commonly used as a laboratory reagent. It serves as a buffering agent, maintaining a stable pH in various experimental and analytical procedures. Sodium citrate is a white, crystalline powder that is highly soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions)
Magnesium chloride hexahydrate, by Thermo Fisher Scientific (2 mentions)
Silicone elastomer base, by Dow (2 mentions)
184 silicone elastomer curing agent, by Dow (2 mentions)
Ultapure agarose, by Thermo Fisher Scientific (2 mentions)
Syber safe dna gel stain, by Thermo Fisher Scientific (2 mentions)
Sybergold dna gel stain, by Thermo Fisher Scientific (2 mentions)
Tween 20 and 10x tbe buffer, by Bio-Rad (2 mentions)
Poly ethylene glycol peg mw 07435, by Merck Group (2 mentions)
Original prusa i3 mk3s 3 d printer, by Prusa Research (2 mentions)
Sterile water for injection, by B. Braun (1 mentions)
Sodium phosphate dibasic, by Mallinckrodt (1 mentions)
Sodium chloride (nacl), by Mallinckrodt (1 mentions)
Sodium acetate, by Mallinckrodt (1 mentions)
Agno3, by Avantor (1 mentions)
4 protocols using sodium citrate
1
Fabrication of Microfluidic Devices
2
Fabrication of Microfluidic Devices
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Deionized water was further purified to 18 MΩ using a NANOpure II Water System (“Nanopure water”; Barnstead Thermolyne Corporation, Ramsey, MN). Polydimethylsiloxane (PDMS) was made from 184 Silicone Elastomer Base and 184 Silicone Elastomer Curing Agent (Dow Chemical Company, Midland, MI) in a 7:1 ratio and cured under vacuum at 50 °C. Sodium chloride, magnesium chloride hexahydrate, RNaseZap™, UltaPure agarose, SyberSafe DNA gel stain, and SyberGold DNA gel stain were purchased from Thermofisher Scientific (Waltham, MA). Sodium citrate was purchased from Mallinckrodt (Staines-upon-Thames, UK), and Tween 20 and 10x TBE buffer from Bio-Rad (Hercules, CA). Poly(ethylene glycol) (PEG) (MW 07435) was purchased from Sigma-Aldrich (Munich, Germany). All 3D printed structures were designed using SolidWorks 2020 Student Edition and printed using 1.75 mm PLA Filament (Hatchbox 3D, Pomona, CA) on an Original Prusa i3 MK3S+ 3-D Printer (Prusa Research, Prague, Czech Republic). All statistical data was analyzed using JMP Pro 16.
3
CHO-Based mAb-A Production Procedure
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The mAb-A is an IgG 1 subtype monoclonal antibody which was produced using conventional biopharmaceutical Chinese hamster ovary (CHO) cells production (Bristol Myers Squibb, Princeton, NJ). Sodium chloride, sodium citrate, sodium phosphate monobasic, sodium acetate, and sodium phosphate dibasic were all purchased (Mallinckrodt, St. Louis, MO). Sterile water for injection (B|BRAUN, Bethlehem, PA) was used in the preparation of all buffers.
4
Biomarker Detection with Streptavidin-Biotin
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The following materials were used as obtained: AgNO 3 (J. T. Baker, Phillipsburg, NJ, USA); Sodium Citrate (Mallinckrodt, St. Louis, MO, USA); EZ-Link Biotin-HPDP (Pierce Thermo Scientific, Waltham, MA, USA); Streptavidin (Invitrogen, Carlsbad, CA, USA); Rabbit polyclonal anti c-Myc IgG (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); Biotin-conjugated goat anti-rabbit IgG antibody (Molecular Probes, Inc., Eugene, OR, USA); Biotin-conjugated rat anti-mouse IFNγ antibody (BD Biosciences, San Diego, CA, USA), Biotin-conjugated rat anti-mouse IL-10 antibody (BD Biosciences, San Diego, CA, USA), Biotin-conjugated rat anti-human TNFα antibody (BioLegend, San Diego, CA, USA).
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