The HAP1 Chronic Myelogenous Leukemia cancer cell line and DNAJA1 knockout cell line was purchased from Horizon Discovery and were cultured in Iscove’s Modified Eagle Medium (Invitrogen) with 10% fetal bovine serum (Gibco), 100 units/ml penicillin, and 100 μg/ml streptomycin at 5% CO2 and 37° C. The LNCaP cancer cell line was purchased from ATCC and were cultured in RPMI-1640 medium (Invitrogen) with 10% fetal bovine serum (FBS, Clontech), 100 units/ml penicillin, and 100 μg/ml streptomycin at 5% CO2 and 37° C.
Iscove s modified eagle medium
Manufactured by Thermo Fisher Scientific
Iscove's Modified Eagle Medium (IMDM) is a cell culture medium developed by Iscove and Melchers. It is designed to support the growth and maintenance of a variety of cell types, including hematopoietic and lymphoid cells. IMDM provides a balanced salt solution with amino acids, vitamins, and other essential nutrients required for cell proliferation and survival in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Takara Bio (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Nylon cell strainer, by BD (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Dnase, by Roche (1 mentions)
2 protocols using iscove s modified eagle medium
1
Cell Culture of Cancer Cell Lines
2
Lung Tissue Digestion and Cell Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole lungs were removed from individual mice, finely cut and digested in Iscove’s modified Eagle medium (IMDM) (Invitrogen) containing 50 U/ml collagenase type I (Invitrogen) and 13 μg/ml DNase (Roche) at 37°C for 90 mins. Digested lung tissue were pushed through 70 or 100 μm nylon cell strainer (Becton Dickson, New Jersey) and subjected to red cell lysis.
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