Single‐cell suspensions from PBMCs were stained with mAb against human CD14 (HCD14; BioLegend), human CD206 (DCN228; Miltenyi Biotec), human/mouse CD11b (M1/70.15.11.5; Miltenyi Biotec), human CD1a (HI149; Miltenyi Biotec), human CD1a (HI149; Miltenyi Biotec), human CD86 (REA968; Miltenyi Biotec), human CCR7 (REA108; Miltenyi Biotec), human CD45 RA (T6D11; Miltenyi Biotec), CD4 (A161A1; BioLegend), and human CD103 (REA803; Miltenyi Biotec). After 20‐minutes incubation at 4°C in the dark, cells were washed and resuspended in PBS for the FACS analysis. For each test, cells were analyzed using FACSVerse Flow Cytometer and DIVA software (BD Biosciences).
Paparo L., Nocerino R., Ciaglia E., Di Scala C., De Caro C., Russo R., Trinchese G., Aitoro R., Amoroso A., Bruno C., Di Costanzo M., Passariello A., Messina F., Agangi A., Napolitano M., Voto L., Gatta G.D., Pisapia L., Montella F., Mollica M.P., Calignano A., Puca A, & Berni Canani R. (2020). Butyrate as a bioactive human milk protective component against food allergy. Allergy, 76(5), 1398-1415.
+ Open protocol