Anti goat igg horseradish peroxidase conjugated antibodies

The chemical was dissolved in dimethyl sulfoxide (DMSO). Chemical reagents were obtained from Sigma (St. Louis, MO, USA). All primary and secondary antibodies used for immunoblotting were used at dilutions of 1:1000 and 1:10,000, respectively. The antibody against p-IRE (Ser 724) was obtained from Thermo Fisher Scientific (Waltham, MA, USA), the antibody against PPARα was purchased from Abcam (Cambridge, UK), and other antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-rabbit IgG-horseradish peroxidase-conjugated antibodies, anti-goat IgG-horseradish peroxidase-conjugated antibodies, and anti-mouse IgG-horseradish peroxidase-conjugated antibodies were obtained from Santa Cruz Biotechnology.

MHY3200 was designed and synthesized by Prof. H. R. Moon at the College of Pharmacy, Pusan National University, Busan, Korea [7 (link)], and dissolved in dimethyl sulfoxide (DMSO). Other chemical reagents were obtained from Sigma–Aldrich (St. Louis, MO, USA). 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Molecular Probes Inc. (Eugene, OR, USA). ECL Western Blotting Detection Reagents, Pierce bicinchoninic acid (BCA) protein assay kit, polyvinylidene difluoride (PVDF) membrane, and Dokdo-MARK™ protein size marker were purchased from GE Healthcare Biosciences (Buckinghamshire, UK), Thermo Fisher Scientific (Rockford, IL, USA), the Millipore Corporation (Bedford, MA, USA), and ElpisBiotech (Daejeon, Korea), respectively. Anti-NOX4, anti-p-Akt (Ser 473), manganese superoxide dismutase (MnSOD), p-p65 (Ser 276), p65, β-actin, transcription factor IIB (TFIIB), and cyclooxygenase-2 (COX-2) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against p-FoxO1 (Ser 256) were purchased from Cell Signaling Technology (Danvers, MA, USA) while anti-rabbit IgG-horseradish peroxidase-conjugated antibodies, anti-mouse IgG-horseradish peroxidase-conjugated antibodies, and anti-goat IgG-horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).