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Tissuetek vip tissue processing system

Manufactured by Sakura Finetek
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Sourced in United States

The Tissue-Tek VIP tissue processing system is a laboratory equipment designed for the automated processing of tissue samples. It facilitates the preparation of tissue samples for further analysis and examination.

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Lab products found in correlation

Blue buffer, by Leica (2 mentions) Axio imager a2, by Zeiss (2 mentions) Rm2135 microtome, by Leica (2 mentions) Selectech staining solutions, by Leica (2 mentions) Haematoxylin, by Leica (2 mentions) Eosin y 515, by Leica (2 mentions) Histogel, by Thermo Fisher Scientific (2 mentions) Zen software, by Zeiss (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)

2 protocols using tissuetek vip tissue processing system

1

Kidney Organoid Histological Processing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney organoids were fixed overnight at 4°C in 2% or 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA), pre-embedded in HistoGel (Thermo Fisher, Carlsbad, CA), then dehydrated and infiltrated with paraffin using a TissueTek VIP tissue processing system (Sakura Finetek USA, Torrance, CA). Planar or transverse 5μm sections were obtained using a Leica RM 2135 microtome (Leica Biosystems, Buffalo Grove, IL). Sections were baked, de-paraffinized and hydrated to water prior to staining following a standard regressive staining protocol using SelecTech staining solutions (Leica Biosystems, Richmond, IL; Haematoxylin #3801570, Define #3803590, Blue Buffer #3802915, and Eosin Y 515 #3801615). Stained slides were serially dehydrated, cleared, and mounted in Permaslip (Alban Scientific Inc, St. Louis, MO #6530B). Images were acquired on a Zeiss Axio Imager A2 with Zeiss Zen software (Zeiss Microscopy, Thornwood, NY).
Lawlor K.T., Vanslambrouck J.M., Higgins J.W., Chambon A., Bishard K., Arndt D., Er P.X., Wilson S.B., Howden S.E., Tan K.S., Li F., Hale L.J., Shepherd B., Pentoney S., Presnell S.C., Chen A.E, & Little M.H. (2020). Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation.. Nature materials, 20(2), 260-271.
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2

Kidney Organoid Histological Processing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney organoids were fixed overnight at 4°C in 2% or 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA), pre-embedded in HistoGel (Thermo Fisher, Carlsbad, CA), then dehydrated and infiltrated with paraffin using a TissueTek VIP tissue processing system (Sakura Finetek USA, Torrance, CA). Planar or transverse 5μm sections were obtained using a Leica RM 2135 microtome (Leica Biosystems, Buffalo Grove, IL). Sections were baked, de-paraffinized and hydrated to water prior to staining following a standard regressive staining protocol using SelecTech staining solutions (Leica Biosystems, Richmond, IL; Haematoxylin #3801570, Define #3803590, Blue Buffer #3802915, and Eosin Y 515 #3801615). Stained slides were serially dehydrated, cleared, and mounted in Permaslip (Alban Scientific Inc, St. Louis, MO #6530B). Images were acquired on a Zeiss Axio Imager A2 with Zeiss Zen software (Zeiss Microscopy, Thornwood, NY).
Lawlor K.T., Vanslambrouck J.M., Higgins J.W., Chambon A., Bishard K., Arndt D., Er P.X., Wilson S.B., Howden S.E., Tan K.S., Li F., Hale L.J., Shepherd B., Pentoney S., Presnell S.C., Chen A.E, & Little M.H. (2020). Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation.. Nature materials, 20(2), 260-271.
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