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Cd11b apc vio770

Manufactured by Miltenyi Biotec
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CD11b-APC-Vio770 is a fluorochrome-conjugated monoclonal antibody used for the detection and analysis of CD11b-positive cells by flow cytometry. CD11b is a cell surface integrin expressed on various immune cells, including monocytes, macrophages, and granulocytes.

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Lab products found in correlation

Ly6c vioblue, by Miltenyi Biotec (2 mentions) F4 80 bv421, by BD (1 mentions) Cd80 v450, by BD (1 mentions) Cd11c apc cy7, by BD (1 mentions) Cd8 v450, by BD (1 mentions) Mhcii fitc, by Miltenyi Biotec (1 mentions) Cd3 apc, by Miltenyi Biotec (1 mentions) Gr 1 apc, by Miltenyi Biotec (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd44 pe cy7, by BD (1 mentions) Facs lsr 2, by BD (1 mentions) Glast pe, by Miltenyi Biotec (1 mentions) Facscanto 2, by BD (1 mentions) Cd3 apc vio770, by Miltenyi Biotec (1 mentions) Ly6g pe vio770, by Miltenyi Biotec (1 mentions) Cd11c pe, by Miltenyi Biotec (1 mentions) Cd4 vioblue, by Miltenyi Biotec (1 mentions) 8 color macsquant, by Miltenyi Biotec (1 mentions) Flowlogic, by Miltenyi Biotec (1 mentions) Nkp46 apc, by Miltenyi Biotec (1 mentions)

4 protocols using cd11b apc vio770

1

Comprehensive Immunophenotypic Analysis by Flow Cytometry

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All flow cytometric analyses were conducting using a Becton Dickinson fluorescence-activated cell sorter (FACS) LSRII and analyzed using FloJo software as previously described.9 (link), 35 (link) JAM-1 surface levels were assessed using an anti-human or anti-mouse JAM-1 antibody (BD Biosciences Pharmingen) as compared to their respective isotype controls. The relative mean fluorescence intensity (MFI) was used to determine JAM-1 receptor surface levels. Cellular apoptosis was assessed using Annexin V-450 and propidium iodide staining (BD Biosciences Pharmingen) in accordance with the manufacturer’s instructions. The percentage of dead cells was quantified using a Live/Dead Fixable Dead Cell Stain Kit (Invitrogen) per the manufacturer’s instructions. The following antibodies were utilized for immune cell phenotypic analysis: CD49b-V450, F4/80-BV421, CD8-V450, CD44-PeCy7, CD62L-allophycocyanin (APC)-Cy7, CD11c-APC-Cy7, CD80-V450 (BD Biosciences Pharmingen), CD335-APC, CD11b-APC-Vio770, Gr1-APC, CD3-APC, CD4-fluorescein isothiocyanate (FITC), and major histocompatibility complex class II (MHCII)-FITC (Miltenyi Biotec).
Jaime-Ramirez A.C., Yu J.G., Caserta E., Yoo J.Y., Zhang J., Lee T.J., Hofmeister C., Lee J.H., Kumar B., Pan Q., Kumar P., Baiocchi R., Teknos T., Pichiorri F., Kaur B, & Old M. (2017). Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. Molecular Therapy Oncolytics, 5, 87-96.
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2

Isolation and identification of microglia and astrocytes

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Microglia or astrocyte cultures were washed in sterile PBS, before scraping and gentle dissociation by pipetting. Cells were resuspended in FACS buffer (1% BSA/PBS) containing Fc Block (1 μg/mL). Cells were stained for CD11b‐APCVio770 and GLAST‐PE (Miltenyi Biotech, UK) for 30 min at 4°C. Cells were washed three times and resuspended in FACS buffer. Flow cytometeric data was collected using a FACSCanto II (BD Biosciences) cell analyzer. Microglia were identified by expression of CD11b and astrocytes by GLAST expression. Flow cytometeric analysis was carried out using FLOWJO version 7.6.5.
Cox D.J., Field R.H., Williams D.G., Baran M., Bowie A.G., Cunningham C, & Dunne A. (2015). DNA sensors are expressed in astrocytes and microglia in vitro and are upregulated during gliosis in neurodegenerative disease. Glia, 63(5), 812-825.
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3

Multicolor Flow Cytometry of Immune Cells

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Antibody reagents were obtained from Miltenyi Biotech: REA clones of antimouse CD3-APC-Vio770 (REA606), CD4-Vioblue (REA 604), CD8a-PE-Vio770 (REA 601), Foxp3-Vio515 (REA 788), NKp46-APC (REA 815), CD11c-PE (REA 754), CD11b-APC-Vio770 (REA 592), Ly6C-Vioblue (REA 796), Ly6G-PE-Vio770 (REA 526). Foxp3 staining was performed using Foxp3 Staining Buffer Set (Miltenyi Biotech).
Flow cytometry analysis was performed using a Miltenyi 8-color MACSQuant, and data were analyzed using Flowlogic (Miltenyi Biotech).
Lantier L., Poupée-Beaugé A., di Tommaso A., Ducournau C., Epardaud M., Lakhrif Z., Germon S., Debierre-Grockiego F., Mévélec M.N., Battistoni A., Coënon L., Deluce-Kakwata-Nkor N., Velge-Roussel F., Beauvillain C., Baranek T., Lee G.S., Kervarrec T., Touzé A., Moiré N, & Dimier-Poisson I. (2020). Neospora caninum: a new class of biopharmaceuticals in the therapeutic arsenal against cancer. Journal for Immunotherapy of Cancer, 8(2), e001242.
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4

Isolation of Murine Cardiac Myeloid Cells

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LV tissue excised from day 0 and day 1 post-MI mice was minced and digested with 600 U/ml collagenase II (Worthington, LS004177, Lot 47E17554B) and 60 U/ml DNase I in Hanks buffered saline solution and filtered through a 30-µm separation filter to generate single-cell suspensions. Red blood cells were lysed (Red Blood Cell Lysis Solution, Miltenyi 130-094-183) and non-specific interactions were blocked with FcR Blocking Reagent (Miltenyi 130-092-575). Cells were stained with the following fluorophore-conjugated antibody panels: CD45-FITC (Miltenyi 130-110-658), CD11b-APC-Vio770® (Miltenyi 130-109-288), F4/80-PerCP-Vio700 (Miltenyi 130-102-161), Ly6C-VioBlue® (Miltenyi 130-111-921), and Ly6G-APC (Miltenyi 130-107-914). Samples were quantified using the MACSQuant Analyzer 10 (Miltenyi). Cell populations were gated on live singlets, with cells from monocyte-derived/macrophage lineage classified as CD45+CD11b+Ly6G− cells.
Mouton A.J., DeLeon-Pennell K.Y., Rivera Gonzalez O.J., Flynn E.R., Freeman T.C., Saucerman J.J., Garrett M.R., Ma Y., Harmancey R, & Lindsey M.L. (2018). Mapping macrophage polarization over the myocardial infarction time continuum. Basic Research in Cardiology, 113(4), 26.
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