The chemicals and reagents that were used, including non-deuterated monopotassium phosphate (KH2PO4), deuterated methanol-d4 (CH3OH-d4), sodium deuterium oxide (NaOD), deuterium oxide (D2O), trimethylsilyl propionic acid-d4 sodium salt (TSP) and α-glucosidase enzyme, were supplied by Merck (Darmstadt, Germany). Liquid nitrogen was purchased from Linde Company (Petaling Jaya, Malaysia).
Deuterated methanol d4 ch3oh d4
Manufactured by Merck Group
Sourced in Germany
Deuterated methanol-d4 (CH3OH-d4) is a laboratory reagent used as a solvent and reference compound in various analytical techniques. It is a stable isotope of methanol, where the hydrogen atoms have been replaced with deuterium atoms. This product is commonly used in nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and other analytical applications that require the use of a deuterated solvent.
Automatically generated - may contain errors
Lab products found in correlation
Trimethylsilyl propionic acid d4 sodium salt tsp, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions)
Griess reagent, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tryple express enzyme, by Thermo Fisher Scientific (1 mentions)
Quercetin, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Recombinant murine ifnγ, by Merck Group (1 mentions)
Penicillin streptomycin antibiotic solution, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
α glucosidase enzyme, by Merck Group (1 mentions)
2 protocols using deuterated methanol d4 ch3oh d4
1
NMR Spectroscopy of Deuterated Compounds
2
Antioxidant and Anti-Inflammatory Assays Protocol
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Ascorbic acid, 2,2-diphenly-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), FeC1 3 6H 2 O, KH 2 PO 4 , Griess reagent, curcumin, lipopolysaccharide (LPS), phosphate buffered saline (PBS), recombinant murine IFN-γ, dimethylsulfoxide (DMSO), phosphate buffer, α-glucosidase enzyme, p-nitrophenyl-α-D-glucopyranose (PNPG), glycine, and quercetin were purchased from Sigma-Aldrich (Hamburg, Germany). Cell culture medium, Dulbecco's Modified Eagle's Medium (DMEM) containing HEPES and L-glutamine for both, with and without phenol red, penicillin-streptomycin antibiotic solutions, foetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and TrypLE Express enzyme were purchased from Gibco/BRL Life Technologies Inc. (Eggenstein, Germany). For NMR analysis, deuterated methanol-d4 (CH 3 OH-d 4 ) and trimethylsilyl propionic (TSP) acid-d4 sodium salt were purchased from Merck (Darmstadt, Germany). For LC-MS analysis, Optima TM LCMS grade solvents including water, acetonitrile (ACN), methanol, formic acid, and ammonium formate were purchased from Fisher Scientific (Geel, Belgium).
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