Cd69 percp cy5.5 560738

Antibodies specific for YY1 (ab12132), p300 (ab10485), histone H3 mono-methylated K4 (ab8895), and DUSP6 (ab76310) from Abcam, anti-acetyl-histone H3 (Lys27) antibodies from EMD Millipore (07–360), and antibodies against pERK (T202/Y204, 4377s), SIRT1 (2310s), and MYB (12319S) from Cell Signaling Technology were used at a dilution of 1 in 1000 for immunoblotting or 1 in 50 for ChIP. β-Actin-specific antibodies were from Sigma-Aldrich (A5441, used at 1 in 5000). Fluorochrome-conjugated antibodies were from BD Biosciences (CD4-V450 (560345), CD8-V500 (560774), CD45RA-AF700 (560673), and CD69-PerCP-Cy5.5 (560738)) and BioLegend (CD62L-PE (304806), and human IL-2 Alexa-Fluor-488 (500314)). All antibodies for flow cytometry were used at 1 in 50. Flow cytometry gating strategies are included in Supplementary Fig. 5.

Antibodies specific for TCF1 (#2203 S) and pERK (T202/Y204, #4377 S) from Cell Signaling Technology, and DUSP6 (ab76310) from Abcam, were used at a dilution of 1 in 1000 for immunoblotting or 1 in 50 for ChIP-PCR. Antibodies specific for β-actin were from Santa Cruz Biotechnology (sc-47778 HRP) and used at 1 in 10000. Fluorochrome-conjugated antibodies were from BD Biosciences including CD4-APC (555349), CD69- PerCP-Cy5.5 (560738) and CD45RA-PE-Cy™7 and BioLegend including CD62L-PE (304806), CD25-APC (302610) and human IL-2 Alexa-Fluor-488 (500314). All antibodies for flow cytometry were used at 1 in 50. PIM1 inhibitor (526521), GSK3β inhibitors, BIO (2’Z,3’E)-6-Bromoindirubin-3ʹ-oxime (361550) and SB216763 (S3442), were from Sigma.