Masshunter workstation version 10

The analyses were carried out using an Agilent 8890 gas chromatograph with an Agilent 7010B mass detector and a Gerstel MPS Robotic injector. The parameters of the analysis method were as follows: column: Agilent 19091S-433UI HP-5ms Ultra Inert, 30 m × 250 μm × 0.25 μm, in constant carrier gas (He) mode of 2 mL/min; inlet chamber: MM Inlet with Agilent 5188–6576 cartridge operating at 1:10 sample split at 250 °C; dispensed sample volume: 1 µL; column temperature program: 60 °C (2 min)–10 °C/min–300 °C (10 min); the injector was working at a temperature of 320 °C; the ion source temperature: 280 °C; temperature of mass analyzers: 150 °C; the mass spectrometer was operated in EI mode at 70 eV; and recording mode: spectrum sweep m/z 40–800. The processing of the obtained results was carried out in the program Agilent MassHunter Workstation version 10.1.

Metalaxyl-M and azoxystrobin were analyzed by high-performance liquid chromatography (Agilent 1290 Infinity II Multisampler, Agilent Technologies, City of Santa Clara, CA, USA) and a tandem triple quadrupole mass spectrometer (6470 Triple Quadrupole LC/MS System, Agilent Technologies, City of Santa Clara, CA, USA) with an electrospray ionization (ESI) source operated in positive ion mode (ESI+). An Agilent EclipsePlusC18 RRHD column (50 mm × 2.1 mm, 1.8 μm) was used for chromatographic separation at a temperature of 40 °C. The mobile phases were acetonitrile (A) and 0.1% (v/v) formic acid aqueous solution (B). The gradient elution procedure is shown in Table S1. The flow was 0.30 mL/min, and the sample injection volume was 1.5 μL. The gas temperature was set at 280 °C and the gas flow rate at 7 L/min for MS detection working conditions. The sheath gas temperature was set at 320 °C and the sheath gas flow was 11 L/min, and the capillary voltages were controlled at 4000 V under positive ion detection mode. Analytes were determined in multiple reaction monitoring (MRM) mode. For instrument control, data acquisition, and processing, MassHunter Workstation version 10.1 (Agilent Technologies, City of Santa Clara, CA, USA) was used. Under the operating conditions given above, the two compounds were quantified based on the acquisition parameters as listed in Table S2.