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3 protocols using potassium chloride (kcl)

1

Biochemical Assay for Oxidative Stress

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Potassium chloride, sodium chloride, sodium bicarbonate, sodium phosphate, calcium chloride were purchased from Central Drug House (CDH), India; glucose were purchased from Fischer Scientific (India). Trichloroacetic acid and ethylenediaminetetraacetic acid were purchased from Qualigens, India; thiobarbituric acid was purchased from Sigma, US. All other chemicals used were of analytical grade. Triple distilled water was used in the experiment.
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2

Biochemical Compound Preparation Protocol

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Potassium chloride, sodium chloride, sodium bicarbonate, sodium phosphate, and calcium chloride were purchased from Central Drug House (CDH), India; glucose was purchased from Fischer scientific (India), trichloroacetic acid, and ethylenediaminetetraacetic acid (EDTA) were purchased from Qualigens, India; thiobarbituric acid was purchased from Sigma, US. All other chemicals used were of analytical grade. Triple distilled water was used in the experiment.
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3

Characterization of Pharmaceutical-Grade MA Formulation

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MA (purity > 99.5%) was received as a kind gift sample from Orchid Pharmaceutical Ltd. (Roorkee, India). Hydroxypropyl methylcellulose-K4M (HPMC-K4M), sodium dodecyl sulfate (SLS), polysorbate 80 (Tween ® 80), hydrochloric acid (HCl), methanol (MeOH), dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), ethanol (C 2 H 5 OH), potassium chloride (KCl), disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 .12H 2 O), N,N-dimethylformamide (DMF), and acetonitrile (CH 3 CN) were commercially obtained from Central Drug House Ltd. (New Delhi, India). The water utilized for all experiments was high-purity/deionized water, which was obtained using a Milli-Q ® water purification unit (Milli-Q Co., Lyon, France). All other materials used were of analytical/pharmaceutical grade.
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