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Muse 1.6 analysis software

Manufactured by Merck Group

The Muse 1.6 Analysis software is a tool developed by Merck Group for the analysis of data generated from various laboratory equipment. The software provides users with a platform to analyze and interpret the data collected from their experiments or studies.

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2 protocols using muse 1.6 analysis software

1

Apoptosis Analysis using Muse Analyzer

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After treatment with SP, SB, SA, SL, BT sup, and CA sup or siRNA transfection for 48 h or BIX for 24 h, the cells were collected and incubated with the Muse Annexin V and Dead Cell Assay kit (MCH100105; Merck) for 20 min at room temperature. For analysis using the Muse™ Caspase 3/7 Kit (MCH100108; Merck), the cells were incubated with caspase 3/7 reagent (Merck) for 30 min in a humidified atmosphere with 5% CO2 at 37 °C. After incubation, the cells were incubated with Caspase 7-AAD (Merck) for 5 min at room temperature. After incubation, ~5 × 104 cells were analyzed using a Muse Cell analyzer (Merck). The FACS results were analyzed using Muse 1.6 Analysis software (Merck).
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2

Apoptosis Assay with Muse Cell Analyzer

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After treatment with BT sup, SP, knockdown of EHMT2 for 48 h or BIX for 24 h, the cells were collected and incubated with the Muse Annexin V and Dead Cell Assay kit (MCH100105; Merck) for 20 min at room temperature. For analysis with the Muse™ Caspase-3/7 Kit (MCH100108; Merck), the cells were incubated with caspase 3/7 reagent (Merck) for 30 min in a humidi ed atmosphere with 5% CO2 at 37˚C. After incubation, the cells were incubated with Caspase 7-AAD (Merck) for 5 min at room temperature. After incubation, ~ 5 × 104 cells were analyzed using a Muse Cell analyzer (Merck). The FACS results were analyzed using Muse 1.6 Analysis software (Merck).
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