Agilent 6224 tof lc ms

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 6224 TOF LC/MS is a high-performance time-of-flight mass spectrometer designed for liquid chromatography applications. It features a broad mass range, high resolving power, and accurate mass measurement capabilities.

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Lab products found in correlation

Uv 8000 spectrophotometer, by Metash (2 mentions) Avance 3 500 mhz nmr spectrometer, by Brucker (1 mentions) P 1010 polarimeter, by Jasco (1 mentions) Chloroform d, by Merck Group (1 mentions) Pyridine d5, by Merck Group (1 mentions) Autopol 1 polarimeter, by Rudolph Research Analytical (1 mentions) Xcalibur atlas gemini ultra diffractometer, by Agilent Technologies (1 mentions) Aviii 500 mhz nmr spectrometer, by Bruker (1 mentions) Cdcl3, by Merck Group (1 mentions) Meoh d4, by Merck Group (1 mentions) J 815 spectropolarimeter, by Jasco (1 mentions) Dionex ultimate 3000 uhplc, by Thermo Fisher Scientific (1 mentions) Adenosine diphosphate (adp), by Promega (1 mentions) Akta pure 15 l instrument, by GE Healthcare (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Advance model, by Bruker (1 mentions) Spectrum one ftir spectrometer, by PerkinElmer (1 mentions)

5 protocols using agilent 6224 tof lc ms

Polymer Degradation Analysis by TOF-MS

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The degradation products from the polymer degradation studies were identified using time-of-flight mass spectrometry (TOF-MS). All mass spectrometric analyses were performed on an Agilent 6224 TOF LC/MS (Agilent Technologies, Palo Alto, CA, USA). The instrument was equipped with an Agilent multimode ion (MMI) source capable of electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). For all experiments, mixed-mode ionization in negative polarity was employed with the range of mass analysis set at 100–3200 m/z. The collected sample solutions were directly introduced into the ion source by flow injection, without a pre-separation step, at a flow rate of 0.22 mL/min via the ESI nebulizer with methanol as the mobile phase (n ≥ 3). The ion source conditions were as follows: capillary voltage, 2500 V; fragmentor voltage, 120 V; skimmer voltage, 60 V; charge voltage, 2000 V; drying gas temperature, 310 °C; drying gas flow rate (N2), 10 L/min; and nebulizer pressure, 45 psig. All data were analyzed with Agilent MassHunter Qualitative Analysis B.07.00.

Yapor J.P., Neufeld B.H., Tapia J.B, & Reynolds M.M. (2018). Biodegradable crosslinked polyesters derived from thiomalic acid and S-nitrosothiol analogues for nitric oxide release. Journal of materials chemistry. B, 6(24), 4071-4081.

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Characterization of Natural Compounds

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Optical rotations were measured on a Jasco P-1010 polarimeter. The ultraviolet (UV) absorption spectra were measured in MeOH on a METASH UV-8000 spectrophotometer. The infrared (IR) spectra were recorded using a Bruker Vector 22 spectrometer (film) and an Avatar 370 Fourier-transform–infrared (FT–IR) spectrometer (KBr disk). NMR spectra were recorded in Chloroform-d (ALDRICH, St. Louis, MO, USA) with tetramethylsilane as an internal standard or Pyridine-d₅ (ALDRICH, St. Louis, MO, USA), using a Bruker AVANCE-III 500 MHz NMR spectrometer (Brucker, Ettlingen, Germany). HR-ESIMS data were obtained on an Agilent 6224 TOF LC/MS (Agilent, Santa Clara, CA, USA) (positive mode; infusion rate: 0.6 mL/min; capillary voltages: 4000 V; nebulizer: 50 psig; drying gas: 10mL/min; gas temperature: 325 °C).
Column chromatography (CC) was performed with silica (100–200 and 200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China).

Li X., Ding W., Wang P, & Xu J. (2018). Two Novel Aspochalasins from the Gut Fungus Aspergillus sp. Z4. Marine Drugs, 16(10), 343.

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Comprehensive Analytical Techniques for Compound Characterization

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Optical rotation was measured on an Autopol I polarimeter (Rudolph Research Analytical, Hackettstown, NJ, USA). The ultraviolet (UV) absorption spectra were measured in MeOH on a METASH UV-8000 spectrophotometer (Shanghai METASH Instruments Co. Ltd., Shanghai, China). The electronic circular dichroism (ECD) was measured on a JASCO J-815 spectropolarimeter (JASCO Co. Tokyo, Japan). The infrared (IR) spectra were recorded using a Bruker Vector 32 spectrometer (Brucker, Karlsruhe, Germany). NMR spectra were recorded in CDCl₃ (ALDRICH, St. Louis, MO, USA) or MeOH-d₄ (ALDRICH, St. Louis, MO, USA) with tetramethylsilane (TMS) as an internal standard, using a Bruker AV III 500 MHz NMR spectrometer. HR-ESIMS data were obtained on an Agilent 6224 TOF LC/MS. Single crystal X-ray crystallography was determined on an Xcalibur Atlas Gemini Ultra diffractometer (Agilent Technologies). Column chromatography (CC) was performed with silica (100–200 and 200–300 mesh, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China).

Li X., Xu J., Wang P, & Ding W. (2022). Novel indole diketopiperazine stereoisomers from a marine-derived fungus Aspergillus sp. Mycology, 14(1), 1-10.

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Biophysical Characterization of Biomolecules

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All reagents were purchased from Sigma-Aldrich unless otherwise specified. FPLC separations were performed using an AKTA pure 15 L instrument (GE Healthcare). HPLC purification and analysis was performed on a Dionex UltiMate 3000 UHPLC+ (Thermo Scientific). For ATPase assays, ATP (Sigma Aldrich) and ADP (Promega) used in these assays were each 99% pure. Compound mass spectrometry analysis was performed on an Agilent 6224 TOF LC/MS, unless otherwise noted.

Hosfelt J., Richards A., Zheng M., Adura C., Nelson B., Yang A., Fay A., Resager W., Ueberheide B., Glickman F, & Lupoli T.J. (2021). An Allosteric Inhibitor of Bacterial Hsp70 Chaperone Potentiates Antibiotics and Mitigates Resistance. Cell chemical biology, 29(5), 854-869.e9.

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Spectroscopic Characterization of Organic Compounds

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UV and IR spectra were measured with Varian Conc. 100 instruments and a Perkin Elmer Spectrum One FTIR spectrometer (Perkin Elmer, USA), respectively. LC-MS/MS was determined on Agilent 6224 TOF-LC/MS using positive and negative mode (Agilent, Santa Clara, USA). The 1 H and 13 C APT NMR spectra were recorded using Bruker Advance Model (500 MHz, 300 MHz for 1 H and 125 MHz, 75 MHz for 13 C, respectively) (Switzerland). The melting points were measured using Melting-Point Apparatus with microscope JM628.

Zain W.Z.W.M., Nawi L., Ahmat N., Osman C.P., & Rukayadi Y. (2021). A New Flavonoid from Malaysian <i>Dipterocarpus cornutus</i>. Indonesian Journal of Chemistry, 21(5), 1132-1132.

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