Immunohistochemical staining was performed as previously described [19 (link)]. Briefly, the slides were incubated overnight with anti-TOM40 antibody (Proteintech) at 4 ℃ in a humidified chamber, and processed with the GTVision III Detection System/Mo&Rb Kit (Gene Tech) to develop color.
Anti tom40 antibody
Manufactured by Proteintech
The Anti-TOM40 antibody is a laboratory reagent used to detect the TOM40 protein. TOM40 is a core component of the translocase of the outer mitochondrial membrane (TOM) complex, which is responsible for the import of proteins into the mitochondria. The antibody can be used in various applications, such as western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of the TOM40 protein.
Automatically generated - may contain errors
Lab products found in correlation
Gtvision 3 detection system mo rb kit, by Gene Tech (1 mentions)
Goat serum, by Beyotime (1 mentions)
Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
2 protocols using anti tom40 antibody
1
Immunohistochemical Staining for TOM40
2
Visualizing Mitochondrial Localization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were cultured in confocal dishes for the stipulated duration, fixed with 4% paraformaldehyde (Sigma-Aldrich) in PBS (Beyotime) for 15 min, and then blocked with 5% goat serum (Beyotime) in PBS for 30 min at room temperature. The cells were incubated overnight with anti-TOM40 antibody (1:200) (Proteintech) at 4 ℃, followed by incubation with Alexa Fluor-488 donkey anti-rabbit IgG (1:500) (Invitrogen) for 1 h at room temperature. After counterstaining with DAPI (Beyotime), the cells were imaged with a Leica TCS SP5 confocal microscope.
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