Ro4929097

DAPT was purchased from Sigma (St Louis, MO, USA). RO4929097 was purchased from Cayman Chemical (Ann Arbor, MI, USA). Noggin was purchased from BioLegend (San Diego, USA). All other chemicals of reagent grade were obtained from Sigma (St Louis, MO, USA).

For heat shock, fish were immersed in a water bath at 37°C for 1 hr before returning to system water at 28°C. For extended periods of heat shock, this was repeated every 6 hr. For inhibition of Tgfb signaling we used two different Tgfb receptor 1 (Alk 5) inhibitors, SB431542 and SB505124 (Fisher Scientific) and to inhibit Notch signaling we used RO4929097 (Cayman). PP2A was inhibited with okadaic acid (Cell Signaling Technology) and p38 MAPK was inhibited with PD169316 (Cayman Chemical). Pharmacological reagents were prepared in DMSO as a 10 mM stock and diluted 1/200 in fish water for immersion or PBS for intravitreal injections. Control fish were treated with vehicle.

The IDO1 inhibitor, INCB-024360, and the γ-secretase inhibitor, Ro-4929097, were purchased from Cayman Chemical Company (Ann Arbor, MI, USA) and dissolved in dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO, USA) as a stock of 50 mM and store at −20 °C. L-kynurenine was purchased from Sigma-Aldrich and was dissolved in 0.5M HCl as a stock of 50 mM and store at −20 °C.