In brief, cell suspension at a density of 2.5 × 104 cell/ml was transferred to multiwall plates and fixed with 2% paraformaldehyde in PBS at room temperature. CD248 (an FLS marker) immunohistochemistry was performed using rabbit anti-CD248 primary antibody (Ab, 1:100 dilution; Abcam, Cambridge) and FITC-conjugated donkey Anti-rabbit IgG secondary antibody (Ab, 1:500 dilution; Jackson ImmunoResearch Laboratories). Non-specific binding was blocked as previously described (Mobasheri et al., 2005 (link)). After 24 h at 4°C, cells were washed three times for 5 min with 0.05% SSC-20 and 0.005% Triton-X100. Slides were finally dipped into distilled water, air dried, and mounted with mounting media (Vectashield with DAPI). Cells were visualized with confocal microscopy.
Fitc conjugated donkey anti rabbit igg secondary antibody ab
Manufactured by Jackson ImmunoResearch
FITC-conjugated donkey Anti-rabbit IgG secondary antibody. This antibody is designed for use in immunochemical techniques.
Automatically generated - may contain errors
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2 protocols using fitc conjugated donkey anti rabbit igg secondary antibody ab
1
Immunohistochemical Analysis of CD248 in FLS
2
Immunofluorescent Detection of CD248 in FLS
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In brief, cell suspension at a density of 2.5x104 cell/ml was transferred to multiwall plates and fixed with 2% paraformaldehyde in PBS at room temperature. CD248 (an FLS marker) immunohistochemistry was performed using rabbit anti-CD248 primary antibody (Ab, 1:100 dilution; AbCam, Cambridge) and FITC-conjugated donkey Anti-rabbit IgG secondary antibody (Ab, 1:500 dilution; Jackson ImmunoResearch Laboratories). Non-specific binding was blocked as previously described (Mobasheri et al. 2005) (link). After 24 hr at 4 ⁰C, cells were washed 3 times for 5 min with 0.05% SSC-20 and 0.005% Triton-X100. Slides were finally dipped into distilled water, air dried and mounted with mounting media (Vectashield with DAPI). Cells were visualised with confocal microscopy.
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