Dulbeccco s modified eagle s media d mem

This study used HEK 293T Cell line, Human Embryonic Kidney cells available from Cat# CRL-3216 ATCC, Manassas, VA, USA. Cells were prepared and cultured as described by ATCC in regular complete culture media: Dulbeccco’s modified Eagle’s media (D-MEM; Gibco Gaithersnurg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) 1% penicillin/streptomycin (Gibco). Cells were cultured in a 10cm dish (Corning, NY, USA) previously coated with Matrigel (Corning, NY, USA) diluted in F-12 media (F-12 Nutrient Mixture, Gibco Gaithersnurg, MD, USA) and propagated at 37 °C in 5% CO₂ as recommended by the manufacturer.
We also used THP-1 cells, human monocyte cells (ATCC, TIB-202) Cells are cultured in T75 flasks in 20 ml complete cell culture medium: Dulbeccco’s modified Eagle’s media (D-MEM; Gibco Gaithersnurg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) 1% penicillin/streptomycin (Gibco). and propagated at 37 °C in 5% CO₂ as recommended by the manufacturer.

HEK 293T, Human Embryonic Kidney cells (CRL-3216, ATCC, Manassas, VA, USA), were prepared and cultured as described by ATCC in regular complete culture media: Dulbeccco’s modified Eagle’s media (D-MEM; Gibco Gaithersnurg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) 1% penicillin/streptomycin (Gibco). Cells were cultured in a 10cm dish (Corning, NY, USA) previously coated with Matrigel (Corning, NY, USA) diluted in F-12 media (F-12 Nutrient Mixture, Gibco Gaithersnurg, MD, USA) as recommended by the manufacturer. During the vesicles collection, a specific media was used and we called it Exo-media: Dulbeccco’s modified Eagle’s media (D-MEM; Gibco Gaithersnurg, MD, USA) supplemented with 10% exosome depleted fetal bovine serum (Exosome-Depleted FBS; Gibco) and 1% penicillin/streptomycin (Gibco). THP-1 cells, human monocyte cells (ATCC, TIB-202) Cells are cultured in T75 flasks in 20 ml complete cell culture medium: Dulbeccco’s modified Eagle’s media (D-MEM; Gibco Gaithersnurg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) 1% penicillin/streptomycin (Gibco). and propagated at 37 °C in 5% CO₂ as recommended by the manufacturer.