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Grdgspc

Manufactured by AAPPTec
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The GRDGSPC is a versatile laboratory equipment designed for general research and development purposes. It provides essential functionalities for scientific experimentation and analysis. The core function of this product is to facilitate controlled and reproducible experimental procedures in a laboratory setting. Further details on the intended use or specific applications are not available.

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Lab products found in correlation

Peg 4acr, by Laysan Bio (1 mentions) Peg 4vs, by Merck Group (1 mentions) Peg 4mal, by Laysan Bio (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Gcrdvpmsmrggdrcg, by GenScript (1 mentions) Penicillin, by Corning (1 mentions) Peg mal, by Laysan Bio (1 mentions) Insulin transferrin selenium, by Corning (1 mentions) Grgdspc, by Biosynth (1 mentions) L glutamine, by Corning (1 mentions)

3 protocols using grdgspc

1

Multicomponent Hydrogel Biomaterials

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PEG-4MAL with 20 kDa molecular weight and ≥90% purity and PEG-4ACR with 21 kDa and >97% were purchased from Laysan Bio (catalogue numbers 4arm-PEG-MAL-20k-1g, 4arm-PEG-ACRL-20k-1g). PEG-4VS with 20 kDa molecular weight and >90% purity was purchased from Sigma Aldrich (catalogue number JKA7025–1G). Peptides (>95% purity) were custom purchased from AAPPTec and included collagen 1 mimic ‘GFOGER’ (GYGGGP(GPP)5GFOGER(GPP)5GPC, where O = hydroxyproline), fibronectin/vitronectin mimic ‘RGD’ (GRGDSPC), VCAM1 mimic ‘REDV’ (GREDVGC), control scrambled peptide (GRDGSPC) and protease-sensitive crosslinker (GCRDVPMS↓MRGGDRCG). The non-degradable crosslinker DTT was purchased from Sigma Aldrich (catalogue number DTT-RO). All components were reconstituted in 0.01 M HEPES (Thermo Fisher, catalogue number 15630080), pH 7.4.
Shah S.B., Carlson C.R., Lai K., Zhong Z., Marsico G., Lee K.M., Félix Vélez N.E., Abeles E.B., Allam M., Hu T., Walter L.D., Martin K.E., Gandhi K., Butler S.D., Puri R., McCleary-Wheeler A.L., Tam W., Elemento O., Takata K., Steidl C., Scott D.W., Fontan L., Ueno H., Cosgrove B.D., Inghirami G., García A.J., Coskun A.F., Koff J.L., Melnick A, & Singh A. (2023). Combinatorial treatment rescues tumour-microenvironment-mediated attenuation of MALT1 inhibitors in B-cell lymphomas. Nature materials, 22(4), 511-523.
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2

PEG-lysostaphin Hydrogel for Antimicrobial Therapy

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Twenty-kilodalton PEG-4MAL macromer (Lysan Bio) was mixed with recombinant lysostaphin protein (AMBI Products LLC) in 100 mM MES buffer, pH 5.5–6.0. Hydrogels were then cross-linked in a one-step reaction by combining PEG-lysostaphin with either the GFOGER peptide, GGYGGP(GPP)5GFOGER(GPP)5GPC (New England Peptide), or the RGD peptide, GRDGSPC (AAPPTEC), VPM cross-linker, GCRDVPMSMRGGDRCG (Genscript), and the bacterial suspension. Bacterial suspensions were prepared by picking individual colonies of bacteria grown on a TSA plate overnight and suspending them in Dulbecco’s PBS supplemented with calcium and magnesium (PBS) to an optical density of 0.20 at 600 nm (MicroScan Turbidity Meter; Seimens) and then diluting this suspension 100-fold. The viable count for all bacterial inocula was determined by plate count on TSA medium. Unless otherwise noted the hydrogels were 4.0% wt/vol 20-kDa PEG-4MAL, 1 mM GFOGER, and 424 U/mL lysostaphin. The amount of VPM cross-linker added was determined stoichiometrically by matching the remaining maleimide groups after accounting for GFOGER or RGD incorporation. After mixing, the hydrogels were allowed to gel for 15 min in a humidified incubator at 37 °C and 5.0% CO2 for in vitro studies or polymerized over the fracture for in vivo studies.
Johnson C.T., Wroe J.A., Agarwal R., Martin K.E., Guldberg R.E., Donlan R.M., Westblade L.F, & García A.J. (2018). Hydrogel delivery of lysostaphin eliminates orthopedic implant infection by Staphylococcus aureus and supports fracture healing. Proceedings of the National Academy of Sciences of the United States of America, 115(22), E4960-E4969.
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3

PEG-based hydrogels for cell encapsulation

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Poly(ethylene glycol)-based hydrogels encapsulating cells and different peptides were prepared using a modified one-step functionalization procedure. Components were resuspended in pH 6.0 10 mM HEPES in Dulbecco’s PBS with Ca2+ and Mg2+ (DPBS++). A premixed solution of the protease-sensitive cross-linker peptide “VPM” (GCRDVPMS-MRGGDRCG) (New England Peptide), hCPCs (10 million cells/mL hydrogel), and nonadhesive ligand ‘RDG' (GRDGSPC) (AAPPTec) or “RGD” (GRGDSPC) (New England Peptide) or “GFOGER” (GGYGGGP(GPP)5GFOGER(GPP)5GPC) (AAPPTec) was mixed with 20 kDa PEG-MAL (Laysan Bio). The final concentration of the RDG/RGD/GFOGER peptide was 1.0 mM, 4% or 5% w/v for PEG and the concentration of VPM (New England Peptide) was equal to the balance maleimide sites remaining after accounting for maleimides theoretically reacting with the 1.0 mM adhesive/scrambled ligands. A schematic of the hydrogel synthesis procedure is shown in Figure 1. The solutions were mixed in a 1 mL syringe barrel as a mold and allowed to gel at 37 °C for 10 min, transferred to a 24 well plate containing 500 μL/well Ham’s F-12 media (without serum) with 1x Insulin-Transferrin-Selenium (Cellgro), 100 U/mL penicillin–0.1 mg/mL streptomyocin cocktail (Cellgro), and 2 mM L-glutamine (Cellgro) (CPC treatment media). Media was changed every 2–3 days.
Bhutani S., Nachlas A.L., Brown M.E., Pete T., Johnson C.T., García A.J, & Davis M.E. (2017). Evaluation of Hydrogels Presenting Extracellular Matrix-Derived Adhesion Peptides and Encapsulating Cardiac Progenitor Cells for Cardiac Repair. ACS biomaterials science & engineering, 4(1), 200-210.
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