Ab52489

Tissues or cell lysates were isolated using cell lysis buffer (Beyotime, Nanjing, China). The membrane was transferred and blocked at 37°C for 1 h, followed by primary antibodies incubation, rabbit anti-MCM7 (1:1000, ab52489), PCNA (1:2000, ab92552) and Ki-67 (1:1000, ab16667), Bax (1:5000; ab32503), Bcl-2 (1:1000, ab59348), Cleaved caspase-3 (1:1000, ab49822), Cleaved caspase-9 (1:1000, ab2324), JAK2 (1:5000, ab108596), p- JAK2 (1:5000, ab32101), STAT3 (1:2000, ab109085), p-STAT3 (1:2000, ab76315) and rabbit anti-β-actin (1:2000, ab8227) were purchased from Abcam (Cambridge, UK), and then re-probed with immunoglobulin G (IgG) complexed to horseradish peroxidase (1:2000, ab6721) antibody. The chemiluminescence kit (Millipore, Germany) was adopted to observe the immune response zone and Image J software (ImageJ Software Inc., USA) was used to quantify the integrated density of each band. Antibodies mentioned before were supplied by Abcam (Cambridge, UK).