The reference standard of glucose and uronic acid were provided by Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Moreover, metformin hydrochloride tablets were purchased from Sino-American Shanghai Squibb Pharmaceuticals Ltd. (Shanghai, China). STZ was obtained from Sigma-Aldrich (Sacramento, CA, USA). All test assay kits and enzyme-linked immunosorbent assay (ELISA) kits were supplied by NanJing JianCheng Bioengineering Institute (Nanjing, China). Tumor necrosis factor-alpha (TNF-α) was obtained from PeproTech Inc. (Cranbury, NJ, USA). Lipopolysaccharides (LPS) were provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). NF-κB p65 (1:2,000), phospho-NF-κB p65(Ser536; 1:2,000), and phospho-IκBα (Ser32; 1:2,000) were purchased from Cell Signaling Technology (Beverly, MA, USA). The radioimmunoprecipitation assay (RIPA) lysis buffer with protease/phosphatase inhibitor cocktail, bicinchoninic acid (BCA) protein assay, anti-β-actin antibodies (1:2,000), and horseradish peroxidase (HRP)-conjugated secondary antibodies was purchased from Beijing ComWin Biotech Co., Ltd. (Beijing, China). All other chemical reagents were of analytical grade.
Bicinchoninic acid bca protein assay
Manufactured by CoWin Biotech
Sourced in China
The Bicinchoninic acid (BCA) protein assay is a colorimetric detection and quantitation method used to measure the total protein concentration in a sample. It relies on the reduction of Cu2+ to Cu+ by protein in an alkaline medium and the subsequent chelation of the Cu+ ions by bicinchoninic acid, resulting in a purple-colored product that absorbs light at 562 nm. The intensity of the color is proportional to the protein concentration, allowing for quantitative analysis.
Automatically generated - may contain errors
Lab products found in correlation
Tumor necrosis factor alpha tnf α, by Thermo Fisher Scientific (2 mentions)
Phospho nf κb p65 ser536, by Cell Signaling Technology (2 mentions)
Phospho iκbα ser32, by Cell Signaling Technology (2 mentions)
Uronic acid, by Yuanye Bio-Technology (2 mentions)
Anti β actin antibodies, by CoWin Biotech (2 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by CoWin Biotech (2 mentions)
Protease phosphatase inhibitor cocktail, by CoWin Biotech (2 mentions)
Lipopolysaccharides (lps), by Solarbio (2 mentions)
Streptozotocin (stz), by Merck Group (2 mentions)
Nf κb p65, by Cell Signaling Technology (2 mentions)
2 protocols using bicinchoninic acid bca protein assay
1
Antidiabetic and Anti-inflammatory Effects
2
Metformin Attenuates Inflammatory Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The reference standard of glucose and uronic acid were provided by Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Moreover, metformin hydrochloride tablets were purchased from Sino-American Shanghai Squibb Pharmaceuticals Ltd. (Shanghai, China). STZ was obtained from Sigma-Aldrich (Sacramento, CA, USA). All test assay kits and enzyme-linked immunosorbent assay (ELISA) kits were supplied by NanJing JianCheng Bioengineering Institute (Nanjing, China). Tumor necrosis factor-alpha (TNF-α) was obtained from PeproTech Inc. (Cranbury, NJ, USA). Lipopolysaccharides (LPS) were provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). NF-κB p65 (1:2,000), phospho-NF-κB p65(Ser536; 1:2,000), and phospho-IκBα (Ser32; 1:2,000) were purchased from Cell Signaling Technology (Beverly, MA, USA). The radioimmunoprecipitation assay (RIPA) lysis buffer with protease/phosphatase inhibitor cocktail, bicinchoninic acid (BCA) protein assay, anti-β-actin antibodies (1:2,000), and horseradish peroxidase (HRP)-conjugated secondary antibodies was purchased from Beijing ComWin Biotech Co., Ltd. (Beijing, China). All other chemical reagents were of analytical grade.
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