Dil (red fluorescent dye, Beyotime, C1991S) was diluted to a concentration of 5 mmol/L with DMSO solution and stored at −20°C. Briefly, every 50 μg (1 μg/μL) of exosomes were added to 1 μL of Dil and incubated for 15 minutes in the dark. To remove excess dye, 3 mL of PBS and Exosome Isolation Reagent were mixed and centrifuged at 1500×g for 30 minutes. Supernatant was then aspirated, and the Dil-labeled exosomes were resuspended in 50 μL of PBS. H9C2 cells were seeded onto a 96-well plate until a confluence of 60%, and then co-cultured with Dil-labeled EVs for two time periods (6 hours, 24 hours). Then, the cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min. The nuclei were stained with 6-diamino-2-phenylindole (DAPI, Beyotime, C1005) for 10 min and subsequently visualized using a fluorescence microscope.
Dil red fluorescent dye
Manufactured by Beyotime
Sourced in China
Dil (red fluorescent dye) is a laboratory reagent used for fluorescence microscopy and flow cytometry applications. It is a lipophilic dye that incorporates into cell membranes, allowing for the visualization and tracking of cells. The dye emits red fluorescence when excited by appropriate wavelengths of light.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dil red fluorescent dye
1
Fluorescent Labeling of Exosomes for Cellular Uptake Analysis
2
Exosome Uptake Visualization in Cardiac Cells
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To evaluate MSC-Exo uptake by H9c2 cells and HUVECs, Dil (red fluorescent dye, C1036, Beyotime, China) was used to label Exos. Then Exos and recipient cells were co-cultured for 6 and 24 h at 37°C, after which they were washed with PBS and fixed with 4% paraformaldehyde for 20 min. Nuclei were then stained with 6-diamidino-2-phenylindole (DAPI) (0.5 g/ml; Beyotime) for 10 min and observed under a confocal microscope.
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