Human her3

Binding kinetics of seribantumab and seribantumab-F(ab’)₂ were evaluated with surface plasmon resonance (SPR) on a Biacore 8k system (Cytiva, Uppsala, Sweden) using a CM5 sensor chip immobilized with human HER3 (Sino Biological, Wayne, PA, USA) and murine ErbB3 (Sino Biological, Wayne, PA, USA). The experiment was performed with multi-cycle injections using five concentrations (100, 50, 25, 12.5, and 6.25 nM) at 25 °C and duplicate surfaces. Sensorgrams were analyzed with a Langmuir 1:1 kinetic model.

The binding affinity of the constructs to human HER3 (Sino Biological, Wayne, PA, USA) and murine mErbB3 (Sino Biological, Wayne, PA, USA) was evaluated with surface plasmon resonance (SPR) on a BIAcore T200 system (GE Healthcare, Uppsala, Sweden) using a CM5 sensor chip immobilized with HSA. The constructs were captured on the HSA surface, followed by multi-cycle injections of five HER3 or mErbB3 concentrations (3.125, 6.25, 12.5, 25, and 50 nM). In addition, the binding affinity to HSA and mouse serum albumin (MSA) was evaluated using the same sensor chip and a multi-cycle setup with three concentrations (3.125, 6.25 and 12.5 nM) of the constructs. All experiments were performed in duplicate. The acquired sensorgrams were analyzed using a Langmuir 1:1 kinetic model.