SUM159 were incubated with doxorubicin (1 µM) for 2 d, after which chemotherapy was removed, and new media added. Photographs were taken using an Olympus inverted microscope with a Canon EOS Rebel T4I. Final magnifications were 4X and 10X. Viable cell number was determined by performing trypan blue stains on cells harvested at 6 h, d1, d2, d3, and d7 post-chemotherapy treatment. Alternatively, DU145 tumor cells were incubated with docetaxel (10 nM). Chemotherapy was removed after 4 d. Viable cell number was determined as above for chemotherapy-treated SUM159 cells.
Eos rebel t4i
Manufactured by Canon
Sourced in Japan
The EOS REBEL T4i is a digital single-lens reflex (DSLR) camera. It features an 18-megapixel CMOS sensor and a DIGIC 5 image processor. The camera is capable of recording full HD 1080p video at 30 frames per second.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using eos rebel t4i
1
Evaluating Cancer Cell Viability After Chemotherapy
2
Topical Endoscopic Fundus Imaging in Mice
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Topical endoscopy fundus imaging was performed, as described previously, 28 with some modifications. An endoscope coupled to a 5-cmelong otoscope with a 3-mm outer diameter (1218AA; Karl Storz, Tuttlingen, Germany) was used. A reflex digital camera with an 18-megapixel CMOS image sensor (EOS REBEL T4i; Canon, Tokyo, Japan) was connected to the endoscope through an adapter. Pupils were dilated using topical 0.5% phenylephrine and 0.5% tropicamide (Mydrin-P; Santen), which were respectively applied 60 and 30 minutes before eye examination. Mice were anesthetized just before the examination, after which their whiskers were shaved, and one drop of 0.4% oxybuprocaine (Nitto Medic, Tokyo, Japan) was applied to each eye. The camera was placed on a platform, and the endoscope was slowly moved toward the mouse. Once the endoscope was in contact with the gel covering the cornea, the photographer adjusted its position by horizontally displacing the tip. Focus and illumination were adjusted during examination of the fundus through the camera.
3
Quantifying Furcal and Bone Areas
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Clinical evaluation was determined by quantifying the furcal area between the mesiobuccal and mesiopalatine roots. The bone area was determined between the mesiobuccal and mesiopalatine roots and below to the bone level found, through the use of ImageJ software. The image was obtained through the EOS Rebel t4i (Canon) equipment at an equivalent distance between all the teeth aiming at a standardization between the measurements made.
4
Non-invasive Fundus Imaging in Mice
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Topical endoscopy fundus imaging (TEFI) was performed as described previously 34 with some modifications. We used an endoscope coupled to a 5-cm-long otoscope with a 3-mm outer diameter (1218AA; Karl Storz, Tuttlingen, Germany). A reflex digital camera with an 18-megapixel CMOS image sensor (EOS REBEL T4i; Canon, Tokyo, Japan) was connected to the endoscope through an adapter. Pupils were dilated with topical 0.5% phenylephrine and 0.5% tropicamide (Mydrin-P; Santen, Osaka, Japan), which were applied 60 minutes and 30 minutes before eye examination, respectively. Mice were anesthetized with an intraperitoneal injection of tribromoethanol (Avertin; Wako, Osaka, Japan) just before the examination, after which their whiskers were shaved, and one drop of 0.4% oxybuprocaine was applied to each eye. The camera was placed on a platform, and the endoscope was slowly moved toward the mouse. Once the endoscope was in contact with the gel covering the cornea, the photographer adjusted its position by horizontally displacing the tip. Focus and illumination were adjusted during examination of the fundus through the camera.
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