Human colorectal cancer cell lines (HCT-15, HCT-116, and DLD1) were purchased from the Korea Cell Lines Bank (KCLB). Professor Mee-Hyun Lee (Dongshin University, KOREA) kindly provided us with the HCT-116 (p53+/+ and p53−/−) cell lines. The human immortalized healthy colon cell CCD-18Co (cultivate in MEM) from KCLB. Cells were cultivated in the following medium (obtained from Gibco by Life Technologies): MEM (CCD-18Co), RPMI 1640 (HCT-15 and DLD1), and McCoy’s 5A (HCT-116 and HCT-116 p53+/+ or p53−/−) supplemented with penicillin–streptomycin (1%) (Solarbio Technology Co., Ltd. Korea) and 10% fetal bovine serum (Biological Industries) in a humidified atmosphere at 37 °C with 5% CO2. All the cells were cytogenetically tested and authenticated before being frozen. Each vial of frozen cells was thawed and maintained in culture for a maximum of 8 passages.
Hct 116
Manufactured by Solarbio
Sourced in China, United States
HCT-116 is a cell line derived from human colorectal carcinoma. It is commonly used in biomedical research as a model system for studying cancer biology and evaluating potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Sw480, by Solarbio (2 mentions)
Ht 29, by Solarbio (2 mentions)
Rpmi 1640 medium, by Solarbio (2 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Ccd 18co, by Thermo Fisher Scientific (1 mentions)
Hct 15, by Solarbio (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Ncm460, by Solarbio (1 mentions)
Ncm460, by BNCC (1 mentions)
Sw620, by Solarbio (1 mentions)
Sw620, by Procell (1 mentions)
Sw480, by Procell (1 mentions)
Hct116, by Procell (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
3 protocols using hct 116
1
Cultivation of Human Colorectal Cancer Cell Lines
2
Colon Cancer Cell Line Culture and Transfection
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Colon cancer HT-29 (catalog numbers: CL-0118), HCT-116 (catalog numbers: CL-0096), SW480 (catalog numbers: CL-0223), and SW620 (catalog numbers: CL-0225B) cells were purchased from Procell (Wuhan, China), and human immortalized colon cells (NCM460, catalog numbers: BNCC339288) were purchased from BNCC (Beijing, China). HT-29 and HCT-116 cell lines were cultured in RPMI‐1640 medium (catalog numbers: 31800, Solarbio, Beijing, China). SW480, SW620, and NCM460 cells were cultured in DMEM culture media (catalog numbers: 12100, Solarbio, Beijing, China). All cell culture media were supplemented with penicillin G (100 μg/mL), streptomycin (100 μg/mL), and 10% fetal bovine serum (FBS; catalog numbers: 164210-50, Wuhan, China), and the cells were grown at 37°C with 5% CO2. The logarithmic growth cells were taken for the experiment. We used Lipofectamine 3000 (Thermo Fisher; catalog numbers: L3000015; Shanghai, China) and GSTO2 siRNA (GenePharma, Shanghai, China; siRNA#1 ID113395, siRNA#2 ID 113396, siRNA#3 ID113397) to make transfections in colon cancer cells based on the provided directions. Western blot (WB) and qRT-PCR were used to detect cell transfection efficiency.
3
Evaluating Isovitexin's Anti-Cancer Potential
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Human colonic epithelial cell (HCEC), and cancer cells HT-29, SW480, HCT116, and LoVo (ATCC, USA) were incubated in RPMI 1640 medium (Solarbio, Beijing, China) with 10% fetal bovine serum (FBS, Hyclone, USA). Cancer cells were cultured at 37 °C with 5% CO 2 until they reached 80% confluence. Cells were collected and re-suspended, and the cell concentration was adjusted to 5 Â 10 3 cells in 96-well plates. After incubation at 37 °C with 5% CO 2 for 24 h, the cells were treated with the indicated concentrations of Isov (0, 2.5, 5, 10, 20, 40 , and 80 lmol/L). Cell Counting Kit-8 (CCK-8, Beyotime, China) was used to measure cell proliferation. Absorbance was measured at 450 nm wavelength. The cell growth inhibition rate and IC 50 values were calculated.
The cells were randomly divided into four groups: control, Isov, insulin-like growth factor-1 (IGF-1), and Isov + IGF-1 groups.
The cells were randomly divided into four groups: control, Isov, insulin-like growth factor-1 (IGF-1), and Isov + IGF-1 groups.
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