The human PXR (hPXR) pTarget plasmid and p3A4-pGL3 have been reported previously (33 (link)). hPXR-pFN10A was constructed by inserting the amplified hPXR cDNA into pFN10A (Promega) at the SgfI/PmeI sites. The pFN11A-based expression plasmid for the PGC1α-LXXLL motif (EAEEPSLLKKLLLAPANTQ) fused to the GAL4 DBD protein (PGC1α-LXXLL-pFN11A) was constructed previously (51 (link)). phRL-TK, phRL-CMV, and pFN21A were purchased from Promega. All mutations or insertions were generated using PrimeSTAR Max DNA Polymerase (Takara Bio) and confirmed by sequencing.
Pfn10a
Manufactured by Promega
The PFN10A is a lab equipment product designed for protein purification. It functions as a 10 mL capacity resin column for efficient separation and purification of proteins from complex samples.
Automatically generated - may contain errors
2 protocols using pfn10a
1
Plasmid Construction for PXR and PGC1α Studies
2
Mammalian Two-Hybrid System for Protein Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein interactions were detected by mammalian two-hybrid system as described previously [22 (link)]. pFN10A(ACT) and pFN11A(BIND) were purchased from Promega. pFN10A(ACT)-human-IRF3 and pFN11A(BIND)-APPV-Npro were transfected into HEK293T cells using Lipofectamine 3000. The empty vector pBIND was served as controls. Cells were incubated for 24 h at 37°C in the presence of 5% CO2, and then the luciferase activity was detected as described above.
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