Recombinant human il 18

Manufactured by MBL Life Science

Sourced in United Kingdom

Recombinant human IL-18 is a cytokine produced using recombinant DNA technology. It is a member of the interleukin family and plays a role in the immune response.

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Lab products found in correlation

Dulbecco s modified eagle s medium, by Lonza (1 mentions) Donkey anti rabbit hrp, by GE Healthcare (1 mentions) Anti lamp1, by Cell Signaling Technology (1 mentions) Biotin labeled anti human il 18, by MBL Life Science (1 mentions)

Spelling variants of this product

IL-18 (37 citations) Recombinant IL-18 (4 citations)

2 protocols using recombinant human il 18

Cytokine Stimulation of A549 Cells

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A549 cells (ref. CCL-185, American Type Culture Collection ATCC, Rockville, MD) were cultured in phenol-red-free Dulbecco's modified Eagle's medium (Lonza, Walkersville, MD) supplemented with 10% (v/v) charcoal/dextran-treated fetal bovine serum to avoid steroid activity (Hyclone, Logan, UT). Dexamethasone was from Calbiochem (Nottingham, UK). Recombinant human IL-1β and IL-1ra were from R&D (Abingdon, UK), and recombinant human IL-18 was from MBL International (Nagoya, Japan). Stock solutions of the reagents were dissolved in either ethanol (DEX) or PBS supplemented with 0.1% human serum albumin (IL-1β, IL-1ra, and IL-18), and working solutions were diluted in complete medium before addition to cell cultures.

Escoll P., Ranz I., Muñoz-Antón N., van-den-Rym A., Alvarez-Mon M., Martínez-Alonso C., Sanz E, & de-la-Hera A. (2015). Sustained Interleukin-1β Exposure Modulates Multiple Steps in Glucocorticoid Receptor Signaling, Promoting Split-Resistance to the Transactivation of Prominent Anti-Inflammatory Genes by Glucocorticoids. Mediators of Inflammation, 2015, 347965.

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Caspase-1 Activation and IL-18 Secretion

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Anti-human caspase-1 (R&D mouse monoclonal IgG2A), mouse IgG2A (kappa) isotype control, anti-caspase-1 (lab-generated rabbit polyclonal caspase-1 antiserum (YK)), YK prebleed rabbit serum, anti-ASC (MBL mouse monoclonal IgG1), anti-ASC (lab-generated rabbit polyclonal ASC antiserum (ASCAR)), anti-human IL-18 (MBL International, rabbit monoclonal, IgG2A), anti- human IL-18 (MBL International, mouse monoclonal, IgG2A), biotin-labeled anti-human IL-18 (MBL International, rat monoclonal, IgG2A), recombinant human IL-18 (MBL International), and anti-LAMP1 (Cell Signaling, D2D11, rabbit IgG). Secondary antibodies were: donkey anti-rabbit-HRP & sheep anti-mouse-HRP (ECL, GE Healthcare, UK).
The source of all agents not specified was from Sigma-Aldrich, US.

Shamaa O.R., Mitra S., Gavrilin M.A, & Wewers M.D. (2015). Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1. PLoS ONE, 10(11), e0142203.

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