Balb c 3t3 cells

Manufactured by American Type Culture Collection

Sourced in United States

BALB/c 3T3 cells are a stable, continuous cell line derived from the embryonic fibroblasts of BALB/c mice. These cells are commonly used as a model system for the study of cellular growth, transformation, and differentiation.

Automatically generated - may contain errors

Lab products found in correlation

10 protocols using balb c 3t3 cells

Cell Culture Protocol for Various Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

L-929 cells (ATCC) were purchased from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (100 units/mL) in a humidified atmosphere at 37 °C with 5% CO₂.
The Chinese Academy of Sciences (Shanghai, China) provided the BALB/c 3T3 cells (ATCC), which were then cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA).
The Chinese Academy of Sciences sold HaCaT cells (ATCC), which were cultivated in DMEM enhanced with 10% FBS. Every flask and plate used for cell culture was bought from Corning (Corning, NY, USA).
The culture medium was changed every 2 days, and cells were dissociated at 80% confluence using trypsin.

Cai X., Zhu J., Luo X., Jin G., Huang Y, & Li L. (2023). A Thermally Stable Recombinant Human Fibronectin Peptide-Fused Protein (rhFN3C) for Faster Aphthous Ulcer (AU) Healing. Bioengineering, 11(1), 38.

+ Open protocol

+ Expand

Culturing MDA-MB-468 and 3T3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDA-MB-468 and Balb/c 3T3 cells were obtained from ATCC^® (Manassas, VA, USA). MDA-MB-468 and 3T3HAS3 cells were authenticated based on short tandem repeat profiles (IDEXX, Columbia, MO, USA) and were negative for mycoplasma (MycoAlert, Lonza, Allendale, NJ, USA). MDA-MB-468 cells were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% fetal bovine serum, and 3T3 lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal calf serum. Co-cultures of MDA-MB-468 and 3T3HAS3 cells were maintained in RPMI supplemented with 10% fetal bovine serum.

Zhao C., Thompson B.J., Chen K., Gao F., Blouw B., Marella M., Zimmerman S., Kimbler T., Garrovillo S., Bahn J., Huang L., Huang Z., Shepard H.M., Rosengren S., Thanos C.D, & Maneval D.C. (2019). The growth of a xenograft breast cancer tumor model with engineered hyaluronan-accumulating stroma is dependent on hyaluronan and independent of CD44. Oncotarget, 10(61), 6561-6576.

+ Open protocol

+ Expand

Fibroblast Culturing and Rat Housing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts (BALB/c-3T3 cells) were obtained from the American Type Culture Collection (Manassas, VA, USA). BALB/c-3T3 cells were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). Cells were grown as suspension cultures and maintained in a humidified atmosphere at 37°C±2°C and 5% CO₂.
Wistar rats (9–11 weeks old, 301–350 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Rats were housed in the following conditions: 25°C±2°C, 30%–70% humidity and 12/12 h light/dark cycle. All animal experiments complied with the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No 8023, revised 1978). Ethics approval was received from the Medical Ethics Committee of Shandong Jining No 1 People’s Hospital (Reference number: SDJNPH20170306001).

You P., Yuan R, & Chen C. (2017). Design and evaluation of lidocaine- and prilocaine-coloaded nanoparticulate drug delivery systems for topical anesthetic analgesic therapy: a comparison between solid lipid nanoparticles and nanostructured lipid carriers. Drug Design, Development and Therapy, 11, 2743-2752.

+ Open protocol

+ Expand

Establishing Mouse and Rat Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts cells (BALB/c-3T3 cells) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Female BALB/c nude mice (6–8 weeks old) and female Sprague Dawley rats (9–11 weeks old) were purchased from Shandong University Laboratory Animal Center (Jinan, People’s Republic of China). The study protocol followed the National Institutes of Health guide for the care and use of laboratory animals. All animal experiments were approved by the Medical Ethics Committee of Jining Medical University (No. JNMU20180646-001).

Li A., Yang F., Xin J, & Bai X. (2019). An efficient and long-acting local anesthetic: ropivacaine-loaded lipid-polymer hybrid nanoparticles for the control of pain. International Journal of Nanomedicine, 14, 913-920.

+ Open protocol

+ Expand

Culturing BALB/c 3T3 Mouse Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts (BALB/c 3T3 cells) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (Gibco, Waltham, MA, USA). Cells were grown as suspension cultures and maintained in a humidified atmosphere at 37±2°C and 5% CO₂.

Yang Y., Qiu D., Liu Y, & Chao L. (2019). Topical anesthetic analgesic therapy using the combination of ropivacaine and dexmedetomidine: hyaluronic acid modified long-acting nanostructured lipid carriers containing a skin penetration enhancer. Drug Design, Development and Therapy, 13, 3307-3319.

+ Open protocol

+ Expand

Isolation and Culture of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

OKT3 hybridoma cells were obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan). BALB/c 3T3 cells, HT29 human colon cancer cells, BC3 hybridoma cells, HB65 hybridoma cells, and Jurkat human T cells were from the American Type Culture Collection (Manassas, VA, USA). 2B4 mouse T cells were kindly provided by Dr. Ming-Zong Lai, Institute of Molecular Biology, Academia Sinica. The B3Z mouse T cell hybridoma was kindly provided by Dr. Ya-Wun Yang, School of Pharmacy, College of Medicine, National Taiwan University. OT-I (15 (link)) and OT-II (16 (link)) transgenic mice were a kind gift from Dr. Nan-Shih Liao, Institute of Molecular Biology, Academia Sinica. Mouse splenic T cells were obtained from BALB/c, C57BL/6, OT-I, or OT-II mice via purification over nylon wool.
Human whole blood, obtained from healthy donors by the Taipei City Blood Bank mentioned already in ethics statement.

Chen B.M., Al-Aghbar M.A., Lee C.H., Chang T.C., Su Y.C., Li Y.C., Chang S.E., Chen C.C., Chung T.H., Liao Y.C., Lee C.H, & Roffler S.R. (2017). The Affinity of Elongated Membrane-Tethered Ligands Determines Potency of T Cell Receptor Triggering. Frontiers in Immunology, 8, 793.

+ Open protocol

+ Expand

MCMV Infection and Antibody Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

B6 TECs, BALB/c TECs or BALB/c 3T3 cells (American Type Culture Collection, Manassas VA) were infected with MCMV-GFP virus at a multiplicity of infection (MOI) of 1 for each cell type. At 72 hours post infection, cells were detached and incubated with either MCMV− or MCMV+ B6 sera, or with transplant sera (D−/R− or D+/R+) at 1:20 dilution for 1 hour on ice. MCMV uninfected cells were also incubated with sera. Cells were washed and stained for viability (Ghost Dye UV450, TONBO Biosciences, San Diego CA), EpCAM-PE (CD326, Clone G8.8, Biolegend, San Diego CA), and anti-mouse IgG1-APC (Clone A85–1, BD Biosciences, San Jose CA). Samples were analyzed using a LSRFortessa (BD Biosciences) and FlowJo v10 software (TreeStar Inc, Ashland OR). Experiments were performed 3 times independently.

Saunders U., Li M., Boddeda S.R., Maher S., Ghere J., Kaptsan I., Dhital R., Velazquez V., Guo L., Chen B., Zeng Q., Schoeb T.R., Cianciolo R, & Shimamura M. (2021). Murine Cytomegalovirus-Induced Complement-fixing Antibodies Deposit in Murine Renal Allografts During Acute Rejection. Transplantation, 105(8), 1718-1729.

+ Open protocol

+ Expand

Mouse Embryonic Fibroblast Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts cells (BALB/c-3T3 cells) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (Gibco, Waltham, MA, USA) at 37°C using a humidified 5% CO₂ incubator.

Zhao X., Sun Y, & Li Z. (2018). Topical anesthesia therapy using lidocaine-loaded nanostructured lipid carriers: tocopheryl polyethylene glycol 1000 succinate-modified transdermal delivery system. Drug Design, Development and Therapy, 12, 4231-4240.

+ Open protocol

+ Expand

Monoclonal Antibodies for PEG Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lipofectamine 2000, Expi293™ Expression System Kit, Opti-MEM, phosphate-buffered saline (PBS), versene, and penicillin-streptomycin were purchased from Invitrogen (Waltham, MA, U.S.A.). Dulbecco’s modified Eagle’s medium (DMEM), bovine calf serum (BCS), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). The restriction enzymes HindIII, ClaI, SfiI, and SalI were purchased from BioLabs, Inc. (Ipswich, MA, U.S.A.). Biotin-conjugated monoclonal mouse anti-PEG antibodies (AGP3-biotin (IgM type) and 3.3-biotin (IgG type)) were provided by Dr. Steve R. Roffler (Academia Sinica, Taipei, Taiwan)^{55 (link)}. FITC-conjugated goat anti-mouse immunoglobulin G Fcγ antibody, horseradish peroxidase (HRP)-conjugated streptavidin, and HRP-conjugated goat anti-mouse IgG Fcγ antibody were purchased from Jackson Immuno Research Labs (West Grove, PA, U.S.A.).
BALB/c 3T3 cells (American Type Culture Collection, Manassas, VA, U.S.A.) were maintained at 37 °C with 5% CO₂ and grown in culture medium containing DMEM, 10% BCS, and 100 units/mL of penicillin and streptomycin (Invitrogen, Calsbad, CA, U.S.A.). Expi293 cells were maintained in Expi293 Expression Medium (Invitrogen, Calsbad, CA, U.S.A.) at 37 °C and 120 rpm.

Chen Y.J., Chen M., Hsieh Y.C., Su Y.C., Wang C.H., Cheng C.M., Kao A.P., Wang K.H., Cheng J.J, & Chuang K.H. (2018). Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates. Scientific Reports, 8, 17868.

+ Open protocol

+ Expand

Purchasing Cell Lines for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lines, including human umbilical vein endothelial HUVECs, human skin fibroblast HFFs, and mouse embryonic fibroblast Balb/c 3T3 cells, were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Cao J., Zhang Y., Zhang P., Zhang Z., Zhang B., Feng Y., Li Z., Yang Y., Meng Q., He L., Cai Y., Wang Z., Li J., Chen X., Liu H., Hong A., Zheng W, & Chen X. (2022). Turning gray selenium into a nanoaccelerator of tissue regeneration by PEG modification. Bioactive Materials, 15, 131-144.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!