All MR scans were performed at 3.0 T using a superconducting Magnex Scientific clinical MR “head only” scanner (Oxford, UK) retrofitted for small animal imaging (Magnex Scientific gradient coil, ID of 21 cm; maximum gradient strength of 400 mT/m) and interfaced with a Direct Drive spectrometer (Varian Inc., Palo Alto, CA). A 30 mm ID “Litzcage” quadrature RF coil (Doty Scientific, Columbia, SC), tuned to 128.8 MHz, was used as a transmit/receive volume coil for imaging. In vivo anatomical images were obtained using a 3D balanced steady-state free precession, (b-SSFP) imaging sequence (T2/T1-weighting) acquired in a sagittal readout. Repetition time (TR), echo time (TE), flip angle and bandwidth (BW) were optimized for best brain image quality. The sequence consisted of TR/TE = 9/4.5 ms, flip angle = 30°, 4 frequencies, 4 signal averages and BW = 40.3 kHz. A FOV of 22.1 mm × 22.1 mm × 22.1 mm with matrix dimensions 156 × 156 × 156 was used to acquire (142 μm)3 isotropic resolution images with full brain coverage (~61 min/scan). During imaging, respiratory rate and internal body temperature of the mice were monitored using an MR compatible physiological monitoring and gating system (SA Instruments Inc., Stony Brook, NY). The temperature of the mouse was maintained at 37±1 °C via temperature control feedback loop controlling an air heating system.
Mr compatible physiological monitoring and gating system
Manufactured by SA Instruments
Sourced in United States
The MR-compatible physiological monitoring and gating system is a device designed for use in magnetic resonance (MR) imaging environments. Its core function is to continuously monitor and record physiological data, such as heart rate and respiration, during MR imaging procedures. The system is specifically engineered to operate safely and effectively within the MR environment.
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Lab products found in correlation
4 protocols using mr compatible physiological monitoring and gating system
1
High-Resolution 3D MRI of Mouse Brain
2
3D MRI Rat Brain Anatomy Imaging
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To define the anatomical volume of interests (VOIs), MR scans were obtained on a 31-cm horizontal-bore Agilent 9.4 T scanner (Agilent Technologies, Santa Clara, CA, USA) using a four-channel array rat head surface coil (Rapid Biomedical GmbH, Rimpar, Germany). The image parameters for the 3D turbo spin-echo (TSE) T2-weighted images were as follows: repetition time (TR) = 2,500 ms; echo time (TE) = 7.45 ms; field of view = 20 mm × 20 mm × 10 mm; matrix size = 128 × 128 × 64; voxel size = 156 μm × 156 μm × 156 μm; echo train length = 43; and scan time = 1 h 54 min 50 s. During imaging, the respiratory rate of the rats was monitored using an MR-compatible physiological monitoring and gating system (SA Instruments, Inc., Stony Brook, NY, USA).
3
High-Resolution Brain Imaging of Mice
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To define the anatomical volumes of interest (VOIs), MR scans were obtained on a 31-cm horizontal-bore Agilent 9.4-T scanner (Agilent Technologies, Santa Clara, CA, United States) using a 2-channel array mouse head surface coil (Rapid Biomedical GmbH, Rimpar, Germany). The image parameters for the turbo spin echo (TSE) 3D T2-weighted image were as follows: repetition time (TR) = 2500 ms; echo time (TE) = 7.45 ms; FOV = 20 mm × 20 mm × 10 mm; matrix size = 128 × 128 × 64; voxel size = 0.156 μm × 0.156 μm × 0.156 μm; echo train length (ETL) = 64; and scan time = 2 h 50 m 50 s. During imaging, the respiratory rate of mice was monitored using an MR-compatible physiological monitoring and gating system (SA Instruments Inc., Stony Brook, NY, United States).
4
Anatomical MRI Imaging of Rat Brain
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To define the anatomical VOIs, MR scans were obtained on a 31-cm horizontal-bore Agilent 9.4 T scanner (Agilent Technologies, Santa Clara, CA, USA) using a four-channel array rat head surface coil (Rapid Biomedical GmbH, Rimpar, Germany). The image parameters for the 3D turbo spin-echo (TSE) T2-weighted images were as follows: repetition time (TR) = 2500 ms; echo time (TE) = 7.45 ms; field of view (FOV) = 20 mm × 20 mm × 10 mm; matrix size = 128 × 128 × 64; voxel size = 156 μm × 156 μm × 156 μm; echo train length (ETL) = 43; and scan time = 1 h 54 min 50 s. During imaging, the respiratory rate of rats was monitored using an MR-compatible physiological monitoring and gating system (SA Instruments Inc., Stony Brook, NY, USA).
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