S aureus atcc 5538

Sodium nitrite, tetrahydrofuran (THF), ethylenediaminetetraacetic acid (EDTA), and the CCK-8 kit were purchased from Sigma Aldrich (St. Louis, MO 63103). Tygon S3^™ E-3603 poly(vinyl chloride) tubing was purchased from Fisher Healthcare (Houston, TX). Acetone was purchased from VWR (Radnor, PA). Carbosil-2080A (CarboSil) was purchased from DSM (Berkeley, CA). Cu NPs (99%, 40–60 nm) were obtained from SkySpring Nanomaterials, Inc. (Houston, TX). Phosphate-buffered saline (PBS), pH 7.4, was used for all in vitro experiments, which contained 138 mM NaCl, 2.7 mM KCl, and 10 mM sodium phosphate. Glutathione, Dulbecco’s modified Eagle’s medium (DMEM), and trypsin-EDTA were purchased from Corning (Manassas, VA 20109). The antibiotic Penicillin-Streptomycin (Pen-Strep) and fetal bovine serum (FBS) were purchased from Gibco-Life Technologies (Grand Island, NY 14072). The bacterial strains P. aeruginosa (ATCC 27853) and S. aureus (ATCC 5538), and mouse 3T3 fibroblast cells (ATCC 1658) were purchased from American Type Culture Collection (ATCC). LB broth was obtained from Fisher Bioreagents (Fair Lawn, NJ). LB Agar was obtained from Difco Laboratories Inc (Detroit, MI). Both the 16-gauge and 14-gauge IV polytetrafluroethylene (PTFE) angiocatheters were purchased from Exel International Co. (Redondo Beach, CA).

Poly(hydroxybuyrate) (PHB) was purchased from Goodfellow (Huntington, England). Fiber-grade polylactic acid (PLA) (NatureWorks 6202D) was purchased from NatureWorks (Minnetonka, MN). Glutathione was purchased from GoldBio (St. Louis, MO). Acetone was purchased from VWR (Radnor, PA). Ethylenediaminetetraacetic acid (EDTA), Dulbecco’s modified Eagle’s medium (DMEM), sodium nitrite, DAPI, and the CCK-8 kit were purchased from Sigma Aldrich (St. Louis, MO). Penicillin-Streptomycin and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island, NY). HCl was purchased from Fisher Scientific (Hampton, NH). Hexafluoro-2-propanol (HFIP) was purchased from Oakwood Scientific (Estill, SC). Phosphate buffered saline (PBS), pH 7.4, composed of 138 mM NaCl, 2.7 mM KCl, and 10 mM sodium phosphate, was used for all in vitro experiments. The lactic dehydrogenase (LDH) kit was purchased from Roche Life Sciences (Indianapolis, IN). The bacteria strain S. aureus (ATCC 5538) and NIH/3T3 mouse fibroblasts (ATCC 1658) were received from American Type Culture Collection (Manassas, VA). Alexa Fluor 488 phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA).