Dhodh 14877 1 ap

Manufactured by Proteintech

DHODH (14877-1-AP) is an antibody product manufactured by Proteintech. It is designed to detect the dihydroorotate dehydrogenase (DHODH) protein. DHODH is an enzyme involved in the de novo biosynthesis of pyrimidine nucleotides.

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Lab products found in correlation

Ab86707, by Abcam (1 mentions) Ab155282, by Abcam (1 mentions) Ab125066, by Abcam (1 mentions) Aicar, by Selleck Chemicals (1 mentions) Parp1 13371 1 ap, by Proteintech (1 mentions) Slc11a2, by Proteintech (1 mentions) F actin, by Yeasen (1 mentions) Tsg101 14497 1 ap, by Proteintech (1 mentions) Gpx4 67763 1 ig, by Proteintech (1 mentions) Sorafenib, by MedChemExpress (1 mentions) Cd9 60232 1 ig, by Proteintech (1 mentions) Gapdh, by CWBIO (1 mentions) Ferrostatin 1, by MedChemExpress (1 mentions)

Close spelling variants of this product

DHODH

2 protocols using dhodh 14877 1 ap

Antibody and Reagent Inventory for Ferroptosis Research

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The antibodies to ACTB (66009-1-Ig), PARP1 (13371-1-AP), DHODH (14877-1-AP), G6PD (25413-1-AP), and solute carrier family 11 member 2 (SLC11A2, also known as DMT1; 20507-AP) were obtained from Proteintech Biotechnology. The antibodies to SLC25A22 (ab137614), GPX4 (ab125066), solute carrier family 7 member 11 (SLC7A11; ab216876), ACSL4 (ab155282), and NCOA4 (ab86707) were purchased from Abcam. The antibodies to SCD (A16429) were obtained from ABclonal.
The antibodies to AMPKα (5831) and phospho-AMPKα (Thr172; 2535) were obtained from Cell Signaling Technology. RSL3 (S8155), ferrostatin-1 (S7243), liproxstatin-1 (S7699), necrostatin-1 (S8037), Z-VAD-FMK (S7023), erastin (S7242), STS (S1421), OA (S4707), and AICAR (S1802) were purchased from Selleck Chemicals. A939572 (T4515) was purchased from TOPSCIENCE. Oxalacetic acid (328-42-7) was purchased from MACKLIN. GSH monoethyl ester (353905) and NAC amide (A0737) were purchased from MilliporeSigma. BODIPY 581/591 C11 (D3861) was obtained from Thermo Fisher Scientific.

Liu Y., Wang Y., Lin Z., Kang R., Tang D, & Liu J. (2023). SLC25A22 as a Key Mitochondrial Transporter Against Ferroptosis by Producing Glutathione and Monounsaturated Fatty Acids. Antioxidants & Redox Signaling, 39(1-3), 166-185.

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Antibody-based Protein Detection and Inhibitor Assessment

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The antibodies used targeted the following proteins (dilutions used are included):
GAPDH (CW0101: immunoblotting, 1:1000) from CWBIOTECH; F-actin (40734ES75: immunofluorescence, 1:100) from YEASEN; U1A (10212: immunoblotting, 1:500) from Proteintech; CD63 (67605-1-Ig: immunoblotting, 1:5000) from Proteintech; CD9 (60232-1-Ig: immunoblotting, 1:5000) from Proteintech; TSG101 (14497-1-AP: immunoblotting, 1:1000) from Proteintech; GPX4 (67763-1-Ig: immunoblotting, 1:5000; IHC, 1:1000) from Proteintech; and DHODH (14877-1-AP: immunoblotting, 1:2000; IHC, 1:100) from Proteintech.
The inhibitors used are as follows:
Sorafenib (HY-10201: 10 μM for the in vitro assay and 30 mg/kg for the in vivo assay) and Ferrostatin-1 (HY-100579: 60 nM for the in vitro assay), and Ferrostatin-1 (HY-100579: 5 mg/kg, intraperitoneal injection for the in vivo assay) were obtained from MedChem Express.

Li X., Yu Q., Zhao R., Guo X., Liu C., Zhang K., Zhang W., Liu J., Yu J., Wang S., Hao Q., Li W., Zhang W., Li M., Zhang Y., Zhang C, & Gao Y. (2022). Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma. Frontiers in Oncology, 12, 898156.

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