COS-1 cells derived from African green monkey kidney were obtained from RIKEN BioResource Center (Ibaraki, Japan). Dulbecco's modified Eagle's medium (high glucose) with l -glutamine, phenol red and sodium pyruvate (D-MEM) was purchased from Wako Pure Chemical Industries, Ltd. Fetal bovine serum (FBS) was purchased from Sigma-Aldrich. Antibiotic-Antimycotic (Anti-Anti), MEM non-essential amino acids (MEM NEAA) and 0.5% trypsin-ethylenediaminetetraacetic acid (EDTA) disodium salt solution were obtained from Life Technologies, Inc. (Carlsbad, CA, USA).
Cells were routinely cultured in D-MEM supplemented with 10% FBS, 1% Anti-Anti and 1% MEM NEAA at 37 °C, in an atmosphere of 5% CO2/95% air under saturating humidity, as described in our previous report (Fujino et al., 2016 (link)). Plasmids used in this study were as described previously (Fujino et al., 2016 (link)).
Cells were routinely cultured in D-MEM supplemented with 10% FBS, 1% Anti-Anti and 1% MEM NEAA at 37 °C, in an atmosphere of 5% CO2/95% air under saturating humidity, as described in our previous report (Fujino et al., 2016 (link)). Plasmids used in this study were as described previously (Fujino et al., 2016 (link)).