The hydrochloride salts of putrescine, spermidine, spermine, and norspermidine were purchased from Sigma (MO, USA). Murashige and Skoog (MS) salts were purchased from Wako (Osaka, Japan).
Murashige and skoog
Manufactured by Fujifilm
Sourced in Japan
Murashige and Skoog is a type of media used for plant tissue culture. It provides the nutrients and growth factors necessary for the in vitro propagation of plant cells, tissues, and organs.
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Lab products found in correlation
3 protocols using murashige and skoog
1
Polyamine Hydrochloride Salt Procurement
2
Arabidopsis T-DNA Insertion Mutant Phenotyping
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Arabidopsis thaliana Columbia-0 (Col-0) was used as the wild type. The T-DNA insertion line, anac032 (SALK_012253) was obtained from the SALK collection and the ABRC. All seeds were sterilised by treating them with 1% bleach and 0.05% Triton X-100 for 5 min, and then washing 3 times with sterilised water. Seeds were germinated on MS (Murashige and Skoog, FUJIFILM Wako Pure Chemical, Osaka, Japan) medium supplemented with 1% sucrose and 1% agarose after two days at 4 °C. Plants were grown vertically in a growth chamber (Panasonic, Osaka, Japan) at 22 °C with a 16 h light/8 h dark cycle. For H2O2 and estradiol treatments, 5-day-old seedlings were transferred onto MS agarose plates containing 500 µM H2O2 and 5 µM estradiol (FUJIFILM Wako Pure Chemical).
SALK_012253 was genotyped using left-border primers on the T-DNA (LB) and right-side (RP) and left-side (LP) primers on the genome. The list of primers that were used in this study is provided in Supplementary TableS2 .
SALK_012253 was genotyped using left-border primers on the T-DNA (LB) and right-side (RP) and left-side (LP) primers on the genome. The list of primers that were used in this study is provided in Supplementary Table
3
Seed Germination Protocols for Rice, Arabidopsis, and Wheat
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Seeds of Oryza sativa L. japonica 'Koshihikari' were sown in nursery trays followed by cultivation for two weeks under 16-h light/8-h dark and 30°C/ 20°C cycles.
Seeds of Arabidopsis thaliana L. (Col-0, CS70000) were sterilized in 90% EtOH.
Then, they were sown on 1/2× Murashige and Skoog medium with 0.25% gellan gum (Wako, Osaka, Japan), 0.05% (v/v) PPM-100 (Plant Cell Technology, Washington, D.C., USA ), 1.25 mM MES-KOH (pH 5.7) and 0.5% sucrose following by cultivation for 21 days at 20°C under 16-h light/8-h dark cycles.
Seeds of Triticum aestivum L. 'Chinese Spring' were sown on 200 μL zirconia beads YTZ-1 (AS-ONE, Osaka, Japan) with 110 μL nuclease-free water in 2 mL microtubes LT-0200 (INA OPTICA, Tokyo, Japan) followed by cultivation for 3 days under 24-h dark and 4°C and 8 days under 24-h light and 20°C.
Seeds of Arabidopsis thaliana L. (Col-0, CS70000) were sterilized in 90% EtOH.
Then, they were sown on 1/2× Murashige and Skoog medium with 0.25% gellan gum (Wako, Osaka, Japan), 0.05% (v/v) PPM-100 (Plant Cell Technology, Washington, D.C., USA ), 1.25 mM MES-KOH (pH 5.7) and 0.5% sucrose following by cultivation for 21 days at 20°C under 16-h light/8-h dark cycles.
Seeds of Triticum aestivum L. 'Chinese Spring' were sown on 200 μL zirconia beads YTZ-1 (AS-ONE, Osaka, Japan) with 110 μL nuclease-free water in 2 mL microtubes LT-0200 (INA OPTICA, Tokyo, Japan) followed by cultivation for 3 days under 24-h dark and 4°C and 8 days under 24-h light and 20°C.
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