T cell subsets were phenotyped in isolated PBMCs or tissue cells by flow cytometry (FACSAria flow cytometer, BD Biosciences, NJ, USA) according to manufacturer’s instructions. The cells were labelled with specific monoclonal antibodies; including anti-CD4-PerCP, anti-CD25-PE, anti- Foxp3-FITC, anti-IL-4-PE and anti-IFN-γ-FITC (all from BD Biosciences, NJ, USA). T cell subsets were selected for detailed phenotypic analysis as follow: (1) Th1 cells: IFN-γ+IL-4- CD4+ T cells; (2) Th2 cells: IFN-γ-IL-4+ CD4+T cells; (3) Treg cells: CD4+CD25+Foxp3+ T cells. A minimum of 106 cells per staining were assessed, with at least 105 events being measured. To measure CCRs in peripheral Treg cells, anti-CCR3, anti-CCR4, anti-CCR5 and anti-CCR8 (R & D Systems, Mineapolis, MN, USA) were used to evaluate corresponding receptor expression in circulating Treg cells of 3 groups.
Anti ccr8
Anti-CCR8 is a laboratory research tool used to detect and study the expression of the CCR8 chemokine receptor. CCR8 is involved in immune cell trafficking and inflammation. This product can be used to identify and quantify CCR8-expressing cells in various biological samples.
Lab products found in correlation
2 protocols using anti ccr8
Multiparametric Flow Cytometry Profiling of T Cell Subsets
T cell subsets were phenotyped in isolated PBMCs or tissue cells by flow cytometry (FACSAria flow cytometer, BD Biosciences, NJ, USA) according to manufacturer’s instructions. The cells were labelled with specific monoclonal antibodies; including anti-CD4-PerCP, anti-CD25-PE, anti- Foxp3-FITC, anti-IL-4-PE and anti-IFN-γ-FITC (all from BD Biosciences, NJ, USA). T cell subsets were selected for detailed phenotypic analysis as follow: (1) Th1 cells: IFN-γ+IL-4- CD4+ T cells; (2) Th2 cells: IFN-γ-IL-4+ CD4+T cells; (3) Treg cells: CD4+CD25+Foxp3+ T cells. A minimum of 106 cells per staining were assessed, with at least 105 events being measured. To measure CCRs in peripheral Treg cells, anti-CCR3, anti-CCR4, anti-CCR5 and anti-CCR8 (R & D Systems, Mineapolis, MN, USA) were used to evaluate corresponding receptor expression in circulating Treg cells of 3 groups.
Chemokine Receptor Interaction Assay
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