Acc antibody

Manufactured by Cell Signaling Technology

The ACC antibody is a tool used in molecular biology research. It is designed to detect the presence of the acetyl-CoA carboxylase (ACC) protein in biological samples. ACC is an enzyme that plays a crucial role in the regulation of fatty acid metabolism. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the ACC protein.

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Lab products found in correlation

Betulin, by Merck Group (1 mentions) Fasn antibody, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 donkey anti mouse a32766, by Thermo Fisher Scientific (1 mentions) Pf 429242, by Merck Group (1 mentions) Srebp1 antibody, by BD (1 mentions) Pgc 1α antibody, by Abcam (1 mentions) Tnf α antibody, by Affinity Biosciences (1 mentions) Mitotracker, by Thermo Fisher Scientific (1 mentions) L aspartate, by Merck Group (1 mentions) Il 6 antibody, by Cell Signaling Technology (1 mentions) E cadherin antibody, by Affinity Biosciences (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Cdna synthesis kit, by Takara Bio (1 mentions)

2 protocols using acc antibody

Lipogenesis Inhibitors SREBP1, FASN, and ACC

Cited 1 time

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The SREBP1 inhibitors, PF429242 and betulin, were purchased from Sigma Aldrich (St. Louis, MO) and Merck (Kenilworth, NJ), respectively. SREBP1 antibody was purchased from BD Biosciences (#557036; San Jose, CA). FASN antibody was purchased from Santa Cruz Biotechnology (SC-55580; Santa Cruz, CA). ACC antibody was purchased from Cell Signaling Technology, Inc (#3662; Beverly, MA). Nile Red (N1142), DAPI (62248) and the secondary antibodies, Alexa Fluor 488-Donkey anti-mouse (A32766) and Alexa Fluor 568-Donkey anti-rabbit (A10042) were purchased from Thermo Fisher Scientific (Waltham, MA). Other reagents and antibodies were the same as described previously^{13 (link), 23 (link)}.

Chen Z., Yu D., Owonikoko T.K., Ramalingam S.S, & Sun S.Y. (2021). Induction of SREBP1 degradation coupled with suppression of SREBP1-mediated lipogenesis impacts the response of EGFR mutant NSCLC cells to osimertinib. Oncogene, 40(49), 6653-6665.

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Comprehensive Metabolic Pathway Analysis

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L-aspartate (Sigma, O7125), Hochest 33342 probe (Thermo, Cat# 62249), Nile red probe (Sigma, N3013), Mito-tracker (Thermo, M7512), TG assay kit (Jiancheng Bio Cat# A110‐2) and DAG assay kit (Uscn Life Science Inc Cat# CEC038Ge), L-aspartate ELISA kit (SinoBestBio, YX-12010 M), FAS antibody (Cell Signaling Technology Cat# 3180, RRID:AB_2100796), ACC antibody (Cell Signaling Technology Cat# 3662, RRID:AB_2219400), IL-6 antibody (Cell Signaling Technology Cat# 12912, RRID:AB_2798059), TNF-α antibody (Affinity Biosciences Cat# AF7014, RRID:AB_2835319), pLKB1 antibody (Santa Cruz Biotechnology Cat# sc-271924, RRID:AB_10610759), PGC-1α antibody (Abcam Cat# ab191838, RRID:AB_2721267), CPT-1β antibody (Affinity Biosciences Cat# DF3904, RRID:AB_2836257), mitochondrial complex antibody, CD68 antibody (Abcam# ab955; RRID: AB_307338). CHOP antibody (Affinity Biosciences Cat# DF6025, RRID:AB_2838000), and E-cadherin antibody (Affinity Biosciences Cat# AF0131, RRID:AB_2833315), TRIzol reagent (Invitrogen, Cat# 15596018), cDNA synthesis kit (Takara, Cat# 6210B). Details of the primer sequences used in the study are shown in Supporting Information.

Rao Y., Kuang Z., Li C., Guo S., Xu Y., Zhao D., Hu Y., Song B., Jiang Z., Ge Z., Liu X., Li C., Chen S., Ye J., Huang Z, & Lu Y. (2021). Gut Akkermansia muciniphila ameliorates metabolic dysfunction-associated fatty liver disease by regulating the metabolism of L-aspartate via gut-liver axis. Gut Microbes, 13(1), 1927633.

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