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Gotaq hot start polymerase and reagents

Manufactured by Promega
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GoTaq Hot Start Polymerase and reagents are a set of products designed for use in the polymerase chain reaction (PCR) process. The GoTaq Hot Start Polymerase is a recombinant Taq DNA polymerase that is inactive at lower temperatures, preventing non-specific amplification. The accompanying reagents provide the necessary components for PCR, including dNTPs, MgCl2, and buffer solutions.

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Lab products found in correlation

Ez 96 dna methylation kit, by Zymo Research (2 mentions) Rnalater stabilization reagent, by Qiagen (2 mentions) Prism 4 for windows, by GraphPad (2 mentions) Dneasy blood and tissue kit, by Qiagen (2 mentions) Dntp mix, by Eurogentec (2 mentions)

Close spelling variants of this product

GoTaq polymerase (396 citations) GoTaq Hot Start Polymerase (92 citations) Hot Start Polymerase (6 citations) GoTaq reagents (3 citations)

2 protocols using gotaq hot start polymerase and reagents

1

DNA Methylation Analysis of Ulcerative Colitis Biopsies

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Endoscopic pinch biopsies from 5 patients with UC and 4 HC were stored in RNAlater stabilization reagent (Qiagen) at −80°C. DNA was extracted from intestinal biopsies and blood leukocytes using the DNeasy Blood and Tissue kit (Qiagen) with 500 ng DNA bisulfite converted using the EZ-96 DNA Methylation Kit (Deep Well Format) (Zymo Research, Irvine, CA). Sequencing and PCR primers were designed using the PyroMark Assay Design Software 2.0 (Qiagen/Biotage, Uppsala, Sweden) (see Table, Supplemental Digital Content 4, http://links.lww.com/IBD/A587). Bisulfite-modified DNA was amplified by PCR using these primers with GoTaq Hot Start Polymerase and reagents (Promega, Madison, WI) and dNTP Mix (Eurogentec, Hampshire, United Kingdom) in a 50-μL reaction volume. A 30 μL aliquot of the PCR product was transferred to a PSQ HS 96 plate (Qiagen/Biotage), and pyrosequencing was performed following the manufacturer's protocol using a PyroMark Q96 Vacuum Prep Workstation and a PyroMark (Qiagen/Biotage) Q96 MD System. DNA methylation levels were quantified using Pyro Q-CpG Software (Qiagen/Biotage). Percentage methylation was analyzed using GraphPad Prism 4 for Windows (GraphPad Software Inc., La Jolla, CA).
Smith P.J., Levine A.P., Dunne J., Guilhamon P., Turmaine M., Sewell G.W., O'Shea N.R., Vega R., Paterson J.C., Oukrif D., Beck S., Bloom S.L., Novelli M., Rodriguez-Justo M., Smith A.M, & Segal A.W. (2014). Mucosal Transcriptomics Implicates Under Expression of BRINP3 in the Pathogenesis of Ulcerative Colitis. Inflammatory Bowel Diseases, 20(10), 1802-1812.
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2

DNA Methylation Analysis of Ulcerative Colitis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Endoscopic pinch biopsies from 5 patients with UC and 4 HC were stored in RNAlater stabilization reagent (Qiagen) at −80°C. DNA was extracted from intestinal biopsies and blood leukocytes using the DNeasy Blood and Tissue kit (Qiagen) with 500 ng DNA bisulfite converted using the EZ-96 DNA Methylation Kit (Deep Well Format) (Zymo Research, Irvine, CA). Sequencing and PCR primers were designed using the PyroMark Assay Design Software 2.0 (Qiagen/Biotage, Uppsala, Sweden) (see Table, Supplemental Digital Content 4, http://links.lww.com/IBD/A587). Bisulfite-modified DNA was amplified by PCR using these primers with GoTaq Hot Start Polymerase and reagents (Promega, Madison, WI) and dNTP Mix (Eurogentec, Hampshire, United Kingdom) in a 50-μL reaction volume. A 30 μL aliquot of the PCR product was transferred to a PSQ HS 96 plate (Qiagen/Biotage), and pyrosequencing was performed following the manufacturer’s protocol using a PyroMark Q96 Vacuum Prep Workstation and a PyroMark (Qiagen/Biotage) Q96 MD System. DNA methylation levels were quantified using Pyro Q-CpG Software (Qiagen/Biotage). Percentage methylation was analyzed using GraphPad Prism 4 for Windows (GraphPad Software Inc., La Jolla, CA).
Smith P.J., Levine A.P., Dunne J., Guilhamon P., Turmaine M., Sewell G.W., O'Shea N.R., Vega R., Paterson J.C., Oukrif D., Beck S., Bloom S.L., Novelli M., Rodriguez-Justo M., Smith A.M, & Segal A.W. (2014). Mucosal Transcriptomics Implicates Under Expression of BRINP3 in the Pathogenesis of Ulcerative Colitis. Inflammatory Bowel Diseases, 20(10), 1802-1812.
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