Paraffin‐embedded tumor tissue microarrays were purchased from Shanghai Outdo Biotech. Brain tumor classification was conducted according to WHO criteria.3 There were 59 specimens on the tissue microarray, including three normal brain tissues, 20 astrocytoma, 34 glioblastoma, and two between astrocytoma and glioblastoma. The clinical characteristics of the cohort are described in Table S1 . The immunohistochemical staining was scored according to the four‐point system (score 0‐3) as previously reported.24 , 25 , 26 A score of 2 or 3 was considered high expression, and the score of 0 or 1 was low expression. The mean immunoreactive score (IRS) was considered as the final IRS (Table S1 ).
Tumor tissue microarrays
Manufactured by Shanghai Outdo Biotech Co.
Tumor tissue microarrays are a collection of small tissue samples from multiple tumor specimens, arranged in a high-density array format on a single slide. This equipment allows for the simultaneous analysis and comparison of multiple tumor samples, facilitating efficient and standardized research on tumor biology and the development of diagnostic and therapeutic tools.
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Lab products found in correlation
2 protocols using tumor tissue microarrays
1
Brain Tumor Tissue Microarray Analysis
2
Quantitative Immunohistochemistry of Cancer Tissues
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Tumor tissue microarrays, purchased from Shanghai Outdo Biotech Company, contain 73 pairs of hepatocellular carcinoma together with matched adjacent normal hepatocellular tissue, 73 pairs of colon cancer together with matched adjacent normal colon tissue, 30 pairs of cervical carcinoma together with matched adjacent normal cervical tissue, and 90 pairs of lung adenocarcinoma together with matched adjacent normal lung tissue and follow-up (range 0–120 months), respectively. Immunohistochemistry was performed by using the avidin-biotin complex method (Vector Labora-tories), including heat-induced antigen-retrieval procedures. Incubation with antibodies against PTEN (1:150; #9188, Cell Signaling), OTUD3 (1:200; HPA028543, Sigma) was carried out at 4 °C for 12 h. All staining was assessed by a quantitative imaging method; the percentage of immunostaining and the staining intensity were recorded. An H-score was calculated using the following formula: H- SCORE = ∑ (PI × I) = (percentage of cells of weak intensity × 1) + (percentage of cells of moderate intensity × 2) + (percentage of cells of strong intensity × 3). PI indicates the percentage of positive cells vs all cells, and I represents the staining intensity.
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