Antibodies used in immunoblotting: COL11A1 (ab64883, Abcam, 1:1000 dilution), ERα (No.8644, CST, 1:1000 dilution), anti-β-actin (60008-1-lg, Proteintech, 1:5000 dilution). Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H + L) was purchased from Jackson Immuno Research. Tamoxifen metabolite 4-hydroxytamoxifen (4-OHT) was purchased from Sigma. Palbociclib HCl (#S1116), abemaciclib mesylate (#S7158), ribociclib HCl (#S5187) and fulvestrant (#S1191) were obtained from Selleck (Shanghai, China).
Normal igg peroxidase conjugated affinipure goat anti rabbit mouse igg h l
Manufactured by Jackson ImmunoResearch
Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L) is a secondary antibody that binds to the heavy and light chains of rabbit or mouse immunoglobulin G (IgG). The antibody is conjugated with horseradish peroxidase (HRP) enzyme, which can be used for detection in various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Fulvestrant, by Selleck Chemicals (2 mentions)
Anti β actin 60008 1 lg, by Proteintech (2 mentions)
Ha no 3724, by Cell Signaling Technology (2 mentions)
Ab64883, by Abcam (1 mentions)
Abemaciclib mesylate, by Selleck Chemicals (1 mentions)
Tamoxifen metabolite 4 hydroxytamoxifen 4 oht, by Merck Group (1 mentions)
Palbociclib hcl, by Selleck Chemicals (1 mentions)
β actin 60008 1 lg, by Proteintech (1 mentions)
Gsk3β 22104 1 ap, by Proteintech (1 mentions)
Gst 10000 0 ap, by Proteintech (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Tubulin 11224 1 ap, by Proteintech (1 mentions)
Cisplatin, by Selleck Chemicals (1 mentions)
Becn1, by Cell Signaling Technology (1 mentions)
P mtor ser2448, by Cell Signaling Technology (1 mentions)
Doxorubicin, by Selleck Chemicals (1 mentions)
Anti zfp36, by Proteintech (1 mentions)
Sqstm1 p62, by Cell Signaling Technology (1 mentions)
Gapdh, by Wuhan Servicebio Technology (1 mentions)
Ki 67 mab 0672, by Beijing Strong Biotechnologies (1 mentions)
4 protocols using normal igg peroxidase conjugated affinipure goat anti rabbit mouse igg h l
1
Western Blot Antibody Validation
2
Immunoblotting Assay with Proteasome and Protein Synthesis Inhibitors
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The proteasome inhibitor MG132 and protein synthesis inhibitor cycloheximide (CHX) were purchased from Selleck. Antibodies used in immunoblotting: eEF2K (ab45168, ab85721, 1:1000) was purchased from Abcam; PD-L1 (17952-1-AP, 1:1000), GSK3β (22104-1-AP, 1:2000), β-actin (60008-1-lg, 1:5000), Flag (66008-3-lg, 1:5000), tubulin (11224-1-AP, 1:5000), GST (10000-0-AP, 1:4000) were purchased from Proteintech; HA (No. 3724, 1:1000), p-GSK3β/S9 (No. 9323, 1:1000) were purchased from Cell Signaling Technologies; phosphoserine monoclonal antibody (mAb) (CSB-MA080235, 1:1000) was purchased from CUSABIO. Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L) was purchased from Jackson Immuno Research.
3
Molecular Mechanisms of Chemotherapeutic Agents
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Paclitaxel (NSC 125 973), cisplatin (s1166), and doxorubicin (s1208) were purchased from Selleck. Actinomycin D (HY‐17559) and rapamycin (HY‐10219) were purchased from MCE. CQ (C6628) was purchased from Sigma. Antibodies used in immunoblotting: mTOR (2972S, 1:1000), p‐mTOR (Ser2448) (5536S, 1:1000), SQSTM1/p62 (88588S, 1:1000), BECN1 (D40C5, 1:1000), LC3 (3868S, 1:1000) were purchased from Cell Signaling Technologies. Anti‐REDD1 (10638‐1‐AP, 1:1000), anti‐AUF1 (12770‐1‐AP, 1:1000), anti‐ZFP36 (12737‐1‐AP, 1:1000), anti‐KHSRP (55409‐1‐AP, 1:1000), anti‐β‐actin (20536‐1‐AP,1:5000), were purchased from Proteintech. GAPDH (GB11002) was purchased from servicebio. LC3 (L7543, 1:2000) was purchased from Sigma. Ki67 (MAB‐0672) was purchased from MXB Biotechnologies. Anti‐H3K27 (ab4729, abcam), anti‐TCF4 (22337‐1‐AP, proteintech) and normal IgG (2729, CST) were used in the CHIP assay. Normal IgG/Peroxidase‐conjugated AffiniPure Goat Anti‐Rabbit/Mouse IgG (H+L) was purchased from Jackson Immuno Research.
4
Dissecting EGFR Signaling Mechanisms
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Tamoxifen and MG132 were purchased from Sigma. Cycloheximide (CHX) was purchased from Amresco. LDN-57444 was purchased from Calbiochem. Fulvestrant and 17β-estradiol (E2) were purchased from Selleck. Antibodies used in immunoblotting: UCH-L1 (No.13179, 1:1000), ERα (No.8644, 1:1000), EGFR (No.4267, 1:1000), HA (No.3724, 1:1000) and pTyr1068-EGFR (No.3777, 1:1000) were purchased from Cell Signaling Technologies. Anti-GST (10000-0-AP, 1:4000), anti-Flag (66008-3-lg, 1:5000), anti-Myc (16286-1-AP, 1:2000), anti-UCH-L1 (66230-1-lg, 1:2000) and anti-β-actin (60008-1-lg, 1:5000) were purchased from Proteintech. Anti-eEF2K (ab45168, 1:1000) was purchased from Abcam. Anti-pThr678-EGFR (orb14895, 1:1000) was purchased from Biorbyt. Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L) was purchased from Jackson Immuno Research.
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