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Tomm70

Manufactured by Santa Cruz Biotechnology
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TOMM70 is a mitochondrial import receptor that acts as a component of the protein translocation complex in the outer mitochondrial membrane. It is responsible for the recognition and subsequent import of proteins destined for the mitochondria.

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Lab products found in correlation

Aldh1a1, by Cell Signaling Technology (1 mentions) Sc 166362, by Santa Cruz Biotechnology (1 mentions) Tomm20, by Cell Signaling Technology (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Aldh1a1, by Santa Cruz Biotechnology (1 mentions) α tublin, by ABclonal (1 mentions) Sc 390545, by Santa Cruz Biotechnology (1 mentions) Psa klk3, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Lc3 1 2, by Cell Signaling Technology (1 mentions) β actin, by ABclonal (1 mentions) Mic10, by Abcam (1 mentions) Star 635p, by Abberior (1 mentions) Star580, by Abberior (1 mentions)

2 protocols using tomm70

1

Comprehensive Protein Expression Analysis

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AR(rabbit monoclonal, 1:1000, #5153), PSA/KLK3(rabbit monoclonal, 1:1000, #5365), TOMM20(rabbit monoclonal, 1:1000, #42,406), ATG5(rabbit monoclonal, 1:1000, #12994S), LC3I/II(rabbit monoclonal, 1:1000, #12,741), Akt (rabbit monoclonal, 1:1000, #4691), Phospho-Akt (Ser473)(rabbit monoclonal, 1:1000, #4060), Phospho-Akt (Thr308)(rabbit monoclonal, 1:1000, #13,038), Nanog(rabbit monoclonal, 1:1000, #4903), Sox2(rabbit monoclonal, 1:1000, #3579), ALDH1A1(rabbit monoclonal, 1:1000, #36671S) and VDAC(rabbit monoclonal, 1:1000, #4661) antibodies were purchased from Cell Signaling Technology (CST). NCAM1(rabbit polyclonal antibody, 1:1000, A7913), β-Actin(mouse polyclonal, 1:5000, AC004) and α-Tublin(mouse polyclonal, 1:5000, AC012) antibodies were purchased from ABclonal Technology (Upper Heyford, UK). NSE (rabbit monoclonal, 1:1000, ab180943) and Syp (Rabbit monoclonal, 1:1000, ab184176) were purchased from Abcam (Shanghai, China). ALDH1A1(mouse monoclonal antibody, 1:500,sc-166362), TOMM70(mouse monoclonal antibody, 1:500, sc-390545),AR(rabbit polyclonal, 1:1000, Sc-815X) antibodies were purchased from Santa cruz. E-cadherin (rabbit polyclonal, 1:1000, GTX100443),BRN2(rabbit polyclonal, 1:1000, GTX114650) and N-cadherin (rabbit polyclonal, 1:1000, GTX127345) were purchased from Genetex.
Yin L., Ye Y., Zou L., Lin J., Dai Y., Fu Y., Liu Y., Peng Y., Gao Y., Fu Y., Qi X., Deng T., Zhang S, & Li X. (2023). AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. Journal of Experimental & Clinical Cancer Research : CR, 42, 204.
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2

Immunofluorescence Staining of Mitochondrial Proteins

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HAP1 cells were fixed with pre‐warmed (37°C) 3.7% paraformaldehyde for 15 min. After fixation, cells were washed three times with PBS, permeabilized with 0.15% Triton X‐100 for 15 min and blocked using 10% goat serum for 15 min followed by incubation with appropriate dilution of primary antibodies for 3 h at room temperature or overnight at 4°C. After washing thrice with PBS, samples were incubated at room temperature with appropriate secondary antibody for 1 h and washed three times with PBS before proceeding for microscopy. For STED super‐resolution imaging, primary antibodies used were against MIC60 (custom‐made, Pineda (Berlin)), MIC10 (Abcam, 84969) and TOMM70 (Santa Cruz Biotechnology, sc‐390545). Goat anti‐rabbit Abberior STAR 635P (Abberior) and goat anti‐mouse Abberior STAR 580 (Abberior) were used as secondary antibodies.
Kondadi A.K., Anand R., Hänsch S., Urbach J., Zobel T., Wolf D.M., Segawa M., Liesa M., Shirihai O.S., Weidtkamp‐Peters S, & Reichert A.S. (2020). Cristae undergo continuous cycles of membrane remodelling in a MICOS‐dependent manner. EMBO Reports, 21(3), e49776.
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